Macciò A, Mantovani G, Turnu E, Artini P, Contu G, Serri F G, Volpe A
Cattedra di Oncologia Ginecologica, Università degli Studi di Cagliari, Italy.
J Assist Reprod Genet. 1993 Nov;10(8):517-22. doi: 10.1007/BF01204362.
Relationships between immune and endocrine systems seem to occur in ovarian follicular fluids (FF). Lymphomonocytes have been found in preovulatory follicles and their specific products, cytokines [interleukin-1 (IL1), IL2], were demonstrated to inhibit steroidogenesis. Ovarian steroids, in turn, reduce the cytokine production from immune-competent cells. In the present study we evaluated whether lymphomonocytes are present in FF, and if both their subset distribution and their IL1 alpha and IL2 secretions, after activation with phytohemagglutinin (PHA), are similar to those of peripheral blood. Interferences of IL1 alpha and IL2 production by FF lymphomonocytes caused by isolated granulosa cells were also evaluated.
The study was performed on 86 FFs obtained from follicles containing mature oocytes that were aspirated at the time of ovum pickup from 27 women undergoing controlled ovarian hyperstimulation with exogenous gonadotropins for an in vitro fertilization (IVF) program [IVF-embryo transfer (IVF-ET) or gamete intrafallopian transfer (GIFT)].
Lymphocytes were found in FF. The distribution of CD8+ and CD3+ lymphocyte subsets is equal to that in peripheral blood, but the percentage of CD11b+, CD16+, and CD4+ cells (its trend) is higher in FF than in peripheral blood. The amount of IL2 and IL1 alpha deriving from PHA-activated FF lymphomonocytes is similar to that of peripheral blood PHA-activated lymphomonocytes. Granulosa cells significantly blunt IL2 and IL1 alpha production by FF lymphomonocytes.
These results suggest that preovulation, a migration of lymphomonocytes from the peripheral compartment to the follicle occurs. However, unfavorable effects of IL2 and IL1 alpha, cytotoxic and antisteroidogenetic activities, are counteracted by the products of granulosa cells. The higher amounts of CD11b+, CD16+, and CD4+ in FF suggest that they could be involved in other immune processes.
免疫和内分泌系统之间的关系似乎存在于卵巢卵泡液（FF）中。已在排卵前卵泡中发现淋巴细胞，并且其特定产物细胞因子[白细胞介素-1（IL1）、IL2]被证明可抑制类固醇生成。反过来，卵巢类固醇会减少免疫活性细胞产生的细胞因子。在本研究中，我们评估了卵泡液中是否存在淋巴细胞，以及在用植物血凝素（PHA）激活后，它们的亚群分布及其IL1α和IL2分泌是否与外周血相似。还评估了分离的颗粒细胞对卵泡液淋巴细胞产生IL1α和IL2的干扰。
本研究对从27名接受体外受精（IVF）程序（IVF-胚胎移植（IVF-ET）或配子输卵管内移植（GIFT））的妇女中，在取卵时从含有成熟卵母细胞的卵泡中抽取的86份卵泡液进行。
在卵泡液中发现了淋巴细胞。CD8 +和CD3 +淋巴细胞亚群的分布与外周血中的分布相同，但卵泡液中CD11b +、CD16 +和CD4 +细胞的百分比（其趋势）高于外周血。PHA激活的卵泡液淋巴细胞产生的IL2和IL1α量与外周血PHA激活的淋巴细胞相似。颗粒细胞显著抑制卵泡液淋巴细胞产生IL2和IL1α。
这些结果表明，在排卵前，淋巴细胞从外周腔室迁移到卵泡中。然而，颗粒细胞的产物抵消了IL2和IL1α的细胞毒性和抗类固醇生成活性等不利影响。卵泡液中较高含量的CD11b +、CD16 +和CD4 +表明它们可能参与其他免疫过程。
