利用含有质粒复制子的mini-Mu-lac克隆大肠杆菌的cysE基因。
Cloning of cysE gene of Escherichia coli with a mini-Mu-lac containing a plasmid replicon.
作者信息
Sirko A E, Hulanicka D M
机构信息
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa.
出版信息
Acta Biochim Pol. 1988;35(1):51-5.
PMID:3140549
Abstract
The restriction map of cysE gene of Escherichia coli was established after cloning a mini-Mu-lac bacteriophage containing a plasmid replicon and recloning on pBR322 vector plasmid. Enzyme assays of transformants indicated the lack of autoregulation of cysE gene.
摘要
在克隆了一个含有质粒复制子的微型Mu - lac噬菌体并在pBR322载体质粒上重新克隆后，构建了大肠杆菌cysE基因的限制酶图谱。对转化子的酶活性测定表明cysE基因缺乏自动调节作用。
Zotero 插件