大肠杆菌中的基因不稳定性
GENETIC INSTABILITY IN ESCHERICHIA COLI.
作者信息
SCHWARTZ N M
出版信息
J Bacteriol. 1965 Mar;89(3):712-7. doi: 10.1128/jb.89.3.712-717.1965.
Schwartz, Norman M. (Yale University, New Haven, Conn.). Genetic instability in Escherichia coli. J. Bacteriol. 89:712-717. 1965.-Results obtained from a transductional analysis demonstrated that an extremely unstable reversion of lac(-) is a suppressor mutation. This mutation suppresses 2 of 22 different lac(-) alleles tested. Five different Hfr strains transferred the suppressor soon after mating and at low frequency. Acridine orange enhanced the segregation of lac(-) clones from the unstable suppressed mutant. Stable suppressed lac(-) clones were produced by the unstable suppressed mutant. Two such stable suppressors were transferred at high frequency and mapped at different chromosomal sites on the basis of time of entry data. One of the stable suppressed lac(-) mutants reverted to the unstable suppressed lac(-) state. It is proposed that the suppressor is carried on an extrachromosomal element. The differences in stability observed may reflect differences in the state of the suppressor-bearing element.
施瓦茨，诺曼·M.（耶鲁大学，康涅狄格州纽黑文）。大肠杆菌中的遗传不稳定性。《细菌学杂志》89:712 - 717。1965年。——转导分析的结果表明，lac(-)的一种极其不稳定的回复是一种抑制突变。该突变抑制了所测试的22个不同lac(-)等位基因中的2个。五个不同的高频重组（Hfr）菌株在交配后不久以低频率转移了这种抑制基因。吖啶橙增强了不稳定抑制突变体中lac(-)克隆的分离。不稳定抑制突变体产生了稳定的抑制lac(-)克隆。两个这样的稳定抑制基因以高频率转移，并根据进入时间数据定位在不同的染色体位点。其中一个稳定的抑制lac(-)突变体回复到不稳定抑制lac(-)状态。有人提出，该抑制基因位于一个染色体外元件上。观察到的稳定性差异可能反映了携带抑制基因元件的状态差异。
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