肺炎支原体在肉汤培养中的增殖
REPLICATION OF MYCOPLASMA PNEUMONIAE IN BROTH CULTURE.
作者信息
LOW I E, EATON M D
出版信息
J Bacteriol. 1965 Mar;89(3):725-8. doi: 10.1128/jb.89.3.725-728.1965.
Abstract
Low, Iolanda E. (Harvard Medical School, Boston, Mass.), and Monroe D. Eaton. Replication of Mycoplasma pneumoniae in broth culture. J. Bacteriol. 89:725-728. 1965.-Reproducible growth curves of Mycoplasma pneumoniae can be obtained with the use of shaking cultures, which allows earlier and higher yields of the organism than stationary or roller cultures. Decreasing oxygen tension clearly decreases the growth of M. pneumoniae to barely detectable levels, with or without glucose or pyruvate as substrates. The use of phenol red as a pH indicator in the medium is helpful in judging the replication of a culture so that log-phase organisms can be harvested in large amounts.
摘要
洛，约兰达·E.（哈佛医学院，马萨诸塞州波士顿），和门罗·D.伊顿。肺炎支原体在肉汤培养中的增殖。《细菌学杂志》89:725 - 728。1965年。——使用振荡培养可获得肺炎支原体可重复的生长曲线，与静置培养或滚管培养相比，振荡培养能使该生物体更早且产量更高。无论有无葡萄糖或丙酮酸作为底物，降低氧张力都会明显降低肺炎支原体的生长至几乎检测不到的水平。在培养基中使用酚红作为pH指示剂有助于判断培养物的增殖情况，以便大量收获对数期的生物体。
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