肺炎支原体在肉汤培养中的增殖
REPLICATION OF MYCOPLASMA PNEUMONIAE IN BROTH CULTURE.
作者信息
LOW I E, EATON M D
出版信息
J Bacteriol. 1965 Mar;89(3):725-8. doi: 10.1128/jb.89.3.725-728.1965.
Abstract
Low, Iolanda E. (Harvard Medical School, Boston, Mass.), and Monroe D. Eaton. Replication of Mycoplasma pneumoniae in broth culture. J. Bacteriol. 89:725-728. 1965.-Reproducible growth curves of Mycoplasma pneumoniae can be obtained with the use of shaking cultures, which allows earlier and higher yields of the organism than stationary or roller cultures. Decreasing oxygen tension clearly decreases the growth of M. pneumoniae to barely detectable levels, with or without glucose or pyruvate as substrates. The use of phenol red as a pH indicator in the medium is helpful in judging the replication of a culture so that log-phase organisms can be harvested in large amounts.
摘要
洛，约兰达·E.（哈佛医学院，马萨诸塞州波士顿），和门罗·D.伊顿。肺炎支原体在肉汤培养中的增殖。《细菌学杂志》89:725 - 728。1965年。——使用振荡培养可获得肺炎支原体可重复的生长曲线，与静置培养或滚管培养相比，振荡培养能使该生物体更早且产量更高。无论有无葡萄糖或丙酮酸作为底物，降低氧张力都会明显降低肺炎支原体的生长至几乎检测不到的水平。在培养基中使用酚红作为pH指示剂有助于判断培养物的增殖情况，以便大量收获对数期的生物体。
相似文献
1
REPLICATION OF MYCOPLASMA PNEUMONIAE IN BROTH CULTURE.肺炎支原体在肉汤培养中的增殖
J Bacteriol. 1965 Mar;89(3):725-8. doi: 10.1128/jb.89.3.725-728.1965.
2
EFFECT OF PH ON HUMAN MYCOPLASMA STRAINS.pH值对人支原体菌株的影响
J Bacteriol. 1965 Feb;89(2):265-70. doi: 10.1128/jb.89.2.265-270.1965.
3
GLUCOSE METABOLISM OF TWO STRAINS OF MYCOPLASMA LAIDLAWII.两株莱氏无胆甾原体的葡萄糖代谢
J Bacteriol. 1963 Oct;86(4):627-36. doi: 10.1128/jb.86.4.627-636.1963.
4
Use of modified New York City medium for growth of Mycoplasma pneumoniae. A preliminary report.使用改良的纽约市培养基培养肺炎支原体。初步报告。
Am J Clin Pathol. 1980 May;73(5):702-5. doi: 10.1093/ajcp/73.5.702.
5
Relation of catalase to substrate utilization by Mycoplasma pneumoniae.肺炎支原体中过氧化氢酶与底物利用的关系。
J Bacteriol. 1968 Apr;95(4):1425-30. doi: 10.1128/jb.95.4.1425-1430.1968.
6
The growth response of Mycoplasma hyopneumoniae and Mycoplasma flocculare based upon ATP-dependent luminometry.基于ATP依赖性发光测定法的猪肺炎支原体和絮状支原体的生长反应。
Vet Microbiol. 1990 Aug;24(2):135-42. doi: 10.1016/0378-1135(90)90061-y.
7
CULTIVATION OF MYCOPLASMA PNEUMONIAE (EATON AGENT).肺炎支原体（伊顿因子）的培养
J Bacteriol. 1963 Aug;86(2):339. doi: 10.1128/jb.86.2.339-339.1963.
8
Culture and metabolism of Mycoplasma gallisepticum.鸡败血支原体的培养与代谢
J Bacteriol. 1962 Feb;83(2):213-8. doi: 10.1128/jb.83.2.213-218.1962.
9
HEMOLYSIN OF MYCOPLASMA PNEUMONIAE.肺炎支原体溶血素
J Bacteriol. 1965 Mar;89(3):813-8. doi: 10.1128/jb.89.3.813-818.1965.
10
In vitro susceptibilities of Mycoplasma pneumoniae, Mycoplasma hominis, and Ureaplasma urealyticum to sparfloxacin and PD 127391.肺炎支原体、人型支原体和解脲脲原体对司帕沙星和PD 127391的体外敏感性
Antimicrob Agents Chemother. 1991 Jun;35(6):1181-5. doi: 10.1128/AAC.35.6.1181.
引用本文的文献
1
A tuneable minimal cell membrane reveals that two lipid species suffice for life.可调谐的最简细胞膜揭示了两种脂质物种就足以维持生命。
Nat Commun. 2024 Nov 8;15(1):9679. doi: 10.1038/s41467-024-53975-y.
2
A tuneable minimal cell membrane reveals that two lipid species suffice for life.一种可调节的最小细胞膜表明，两种脂质种类就足以维持生命。
bioRxiv. 2024 Oct 18:2023.10.24.563757. doi: 10.1101/2023.10.24.563757.
3
Genome sequence of strain A5969.菌株A5969的基因组序列。
Microbiol Resour Announc. 2024 Nov 12;13(11):e0081324. doi: 10.1128/mra.00813-24. Epub 2024 Oct 14.
4
Whole-Genome Sequencing of Three M. gallisepticum Isolates Similar to the 6/85 Vaccine Strain.三株与6/85疫苗株相似的鸡毒支原体分离株的全基因组测序
Microbiol Resour Announc. 2023 Jul 18;12(7):e0033923. doi: 10.1128/mra.00339-23. Epub 2023 Jun 22.
5
Complete Genome Sequences of Three Mycoplasma gallisepticum 6/85-like Isolates.三株鸡毒支原体6/85样分离株的全基因组序列
Microbiol Resour Announc. 2022 Aug 18;11(8):e0024422. doi: 10.1128/mra.00244-22. Epub 2022 Aug 2.
6
Model-driven design allows growth of Mycoplasma pneumoniae on serum-free media.模型驱动设计允许肺炎支原体在无血清培养基中生长。
NPJ Syst Biol Appl. 2020 Oct 23;6(1):33. doi: 10.1038/s41540-020-00153-7.
7
Complete Genome Sequences of Two Vaccine Strains and One Field Isolate of Mycoplasma gallisepticum.鸡毒支原体两种疫苗株和一株田间分离株的全基因组序列
Microbiol Resour Announc. 2019 Dec 5;8(49):e01237-19. doi: 10.1128/MRA.01237-19.
8
Complete Genome Sequences of Two Mycoplasma gallisepticum F-Strain Variants.两种鸡毒支原体F株变体的全基因组序列
Microbiol Resour Announc. 2019 Aug 15;8(33):e00485-19. doi: 10.1128/MRA.00485-19.
9
Effect of Medium on H(2)O(2) Levels and Peroxidase-Like Activity by Mycoplasma pneumoniae.肺炎支原体对 H(2)O(2)水平和过氧化物酶样活性的介质影响。
Infect Immun. 1971 Jan;3(1):80-6. doi: 10.1128/iai.3.1.80-86.1971.
10
MYCOPLASMA SPECIES OF MAN.人类支原体物种
Bacteriol Rev. 1965 Jun;29(2):185-221. doi: 10.1128/br.29.2.185-221.1965.
本文引用的文献
1
Hemolysis in identifying Eaton's pleuro-pneumonia-like organism.溶血在伊顿氏类胸膜肺炎微生物鉴定中的作用
Science. 1963 Jan 4;139(3549):55. doi: 10.1126/science.139.3549.55.
2
Hemolyin production as an aid in the identification and quantitation of Eaton agent (Mycoplasma pneumoniae).溶血素产生在伊顿因子（肺炎支原体）鉴定和定量中的作用
Am J Hyg. 1963 Jan;77:122-8. doi: 10.1093/oxfordjournals.aje.a120290.
3
Growth of Eaton PPLO in broth and preparation of complement fixing antigen.伊顿氏菌质体在肉汤中的生长及补体结合抗原的制备
Proc Soc Exp Biol Med. 1962 Aug-Sep;110:884-9. doi: 10.3181/00379727-110-27681.
4
Growth on artificial medium of an agent associated with atypical pneumonia and its identification as a PPLO.一种与非典型肺炎相关的病原体在人工培养基上的生长及其被鉴定为类胸膜肺炎微生物。
Proc Natl Acad Sci U S A. 1962 Jan 15;48(1):41-9. doi: 10.1073/pnas.48.1.41.
5
The pathway for glucose oxidation by Asterococcus mycoides, the organism of bovine pleuropneumonia.牛传染性胸膜肺炎病原体——蕈状星状球菌进行葡萄糖氧化的途径。
Aust J Biol Sci. 1954 Feb;7(1):37-46.
6
The breakdown of pyruvate by Asterococcus mycoides, the organism of bovine pleuropneumonia.牛传染性胸膜肺炎病原体——蕈状星状菌对丙酮酸的分解代谢
Aust J Biol Sci. 1954 Feb;7(1):31-6.
7
The breakdown of carbohydrates by Asterococcus mycoides, the organism of bovine pleuropneumonia.牛传染性胸膜肺炎病原体——蕈状星形球菌对碳水化合物的分解作用。
Aust J Biol Sci. 1954 Feb;7(1):18-30.
Zotero 插件