SOMERSON N L, PURCELL R H, TAYLOR-ROBINSON D, CHANOCK R M
J Bacteriol. 1965 Mar;89(3):813-8. doi: 10.1128/jb.89.3.813-818.1965.
Somerson, Norman L. (National Institute of Allergy and Infectious Diseases, Bethesda, Md.), Robert H. Purcell, David Taylor-Robinson, and Robert M. Chanock. Hemolysin of Mycoplasma pneumoniae. J. Bacteriol. 89:813-818. 1965.-Discrete colonies of Mycoplasma pneumoniae or M. laidlawii on agar produced complete (beta) lysis of guinea pig erythrocytes. Crowding of colonies on agar plates, omission of yeast extract from the medium, and incubation under reduced oxygen tension inhibited hemolysin production. Colonies which were not viable after ultraviolet radiation or heating at 56 C did not produce hemolysin. In addition, hemolysis was suppressed in old cultures in which viability was diminished, and was eliminated when colonies were removed from the agar surface by micromanipulation. The hemolysin passed through a viscose dialysis membrane. These findings suggest that the hemolysin is labile, continuously released by the colonies, of low molecular weight, and probably nonprotein in nature.
索默森，诺曼·L.（国立过敏与传染病研究所，马里兰州贝塞斯达），罗伯特·H.珀塞尔，大卫·泰勒 - 罗宾逊，以及罗伯特·M.查诺克。肺炎支原体的溶血素。《细菌学杂志》89:813 - 818。1965年。 - 肺炎支原体或莱氏无胆甾原体在琼脂上的离散菌落可使豚鼠红细胞发生完全（β）溶血。琼脂平板上菌落密集、培养基中省略酵母提取物以及在低氧张力下培养均会抑制溶血素的产生。经紫外线辐射或56℃加热后无活力的菌落不产生溶血素。此外，在活力降低的陈旧培养物中溶血作用受到抑制，当通过显微操作将菌落从琼脂表面移除时溶血作用消失。溶血素可透过粘胶透析膜。这些发现表明溶血素不稳定，由菌落持续释放，分子量低，且可能本质上不是蛋白质。
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