藤黄八叠球菌的脂质。3. 复合脂质的组成。
LIPIDS OF SARCINA LUTEA. 3. COMPOSITION OF THE COMPLEX LIPIDS.
作者信息
HUSTON C K, ALBRO P W, GRINDEY G B
出版信息
J Bacteriol. 1965 Mar;89(3):768-75. doi: 10.1128/jb.89.3.768-775.1965.
Huston, Charles K. (Fort Detrick, Frederick, Md.), Phillip W. Albro, and Gerald B. Grindey. Lipids of Sarcina lutea. III. Composition of the complex lipids. J. Bacteriol. 89:768-775. 1965.-The complex lipids from a strain of Sarcina lutea were isolated and separated into fractions on diethylaminoethyl cellulose acetate and silicic acid columns. These fractions were monitored in several thin-layer chromatography systems. The various lipid types were characterized by their behavior in thin-layer systems and by an analysis of their hydrolysis products. The fatty acid composition of the column fractions was determined by gas-liquid chromatography. A number of components (13) were separated by thin-layer chromatography and characterized. The major components were polyglycerol phosphatide (17.0%), lipoamino acids (15.1%), phosphatidyl glycerol (13.8%), and an incompletely characterized substance (15.0%). Minor constituents included phosphatidyl inositol (5.5%), phosphatidic acid (4.2%), phosphatidyl serine (2.0%), and phosphatidyl choline (1.0%). No phosphatidyl ethanolamine was observed.
休斯顿，查尔斯·K.（马里兰州弗雷德里克市迪特里克堡），菲利普·W.阿尔布罗，杰拉尔德·B.格林迪。藤黄八叠球菌的脂质。III.复合脂质的组成。《细菌学杂志》89:768 - 775。1965年。——从一株藤黄八叠球菌中分离出复合脂质，并在二乙氨基乙基纤维素醋酸酯柱和硅酸柱上进行分离。这些馏分在几种薄层色谱系统中进行监测。通过它们在薄层系统中的行为以及对其水解产物的分析来表征各种脂质类型。通过气液色谱法测定柱馏分的脂肪酸组成。通过薄层色谱法分离并表征了许多成分（13种）。主要成分是聚甘油磷脂（17.0%）、脂氨基酸（15.1%）、磷脂酰甘油（13.8%）和一种未完全表征的物质（15.0%）。次要成分包括磷脂酰肌醇（5.5%）、磷脂酸（4.2%）、磷脂酰丝氨酸（2.0%）和磷脂酰胆碱（1.0%）。未观察到磷脂酰乙醇胺。
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