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通过气相色谱法和气相色谱-质谱联用技术鉴定藤黄八叠球菌中的脂肪酸和脂肪烃。

Identification of fatty acids and aliphatic hydrocarbons in Sarcina lutea by gas chromatography and combined gas chromatography-mass spectrometry.

作者信息

Tornabene T G, Gelpi E, Oró J

出版信息

J Bacteriol. 1967 Aug;94(2):333-43. doi: 10.1128/jb.94.2.333-343.1967.

Abstract

The composition and nature of the fatty acids and hydrocarbons of Sarcina lutea were elucidated by gas chromatography and by combined gas chromatography-mass spectrometry. The distribution of fatty acids found in S. lutea showed two families of pairs, or dyads, of saturated monocarboxylic acids (C12-C18) with and without methyl branching. These pairs of fatty acids showed a pattern of iso and anteiso structures for C13, C15, and C17, and iso and normal structures for C12, C14, and C16. Only the C18 showed unsaturation. The distribution of hydrocarbons in the range C22-C29 showed two families of tetrads of unsaturated aliphatic hydrocarbons all showing methyl branching. Each tetrad was composed of four isomers identified as two iso olefins and two anteiso olefins. The only difference between the tetrads pertaining to different families was found in the relative gas chromatographic retention times of the last two components of each group.

摘要

通过气相色谱法以及气相色谱 - 质谱联用技术,阐明了藤黄八叠球菌中脂肪酸和碳氢化合物的组成与性质。藤黄八叠球菌中脂肪酸的分布显示出两类饱和单羧酸(C12 - C18)对或二元组,有甲基分支和无甲基分支的情况。这些脂肪酸对在C13、C15和C17处呈现异和反异结构模式,在C12、C14和C16处呈现异和正结构模式。只有C18显示出不饱和性。C22 - C29范围内碳氢化合物的分布显示出两类不饱和脂肪族碳氢化合物四元组,均呈现甲基分支。每个四元组由四个异构体组成,被鉴定为两个异烯烃和两个反异烯烃。不同族的四元组之间唯一的差异在于每组最后两个组分的相对气相色谱保留时间。

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