Cole B C, Ward J R, Martin C H
J Bacteriol. 1968 Jun;95(6):2022-30. doi: 10.1128/jb.95.6.2022-2030.1968.
Various methods for the detection of hemolysin production by Mycoplasma species were compared. Inoculation of blood-agar by the push-block method and by use of concentrated mycoplasma cell suspensions was compared with the agar-overlay technique. The preferred method was direct surface inoculation of concentrated suspensions onto the blood-agar. Among the conditions tested, refrigeration of 48-hr cultures gave the best results. A wide variety of mycoplasma species were tested for hemolytic activity towards rabbit, sheep, guinea pig, duck, and chicken bloods. Guinea pig erythrocytes were found to be the most susceptible to lysis by mycoplasma, and rabbit erythrocytes were found to be the least susceptible. A sensitive technique for the detection of peroxide production by mycoplasma strains, employing agar containing benzidine and sheep blood, was used. With this method, peroxide production could be correlated with hemolysis on blood-agar. Peroxidase and catalase inhibited both the benzidine reaction and hemolysis. It was concluded that the major hemolysin of the Mycoplasma species examined is a peroxide.
对检测支原体产生溶血素的各种方法进行了比较。将推块法接种血琼脂以及使用浓缩支原体细胞悬液接种血琼脂与琼脂覆盖技术进行了比较。首选方法是将浓缩悬液直接表面接种到血琼脂上。在所测试的条件中，对48小时培养物进行冷藏得到的结果最佳。对多种支原体菌株针对兔、羊、豚鼠、鸭和鸡血的溶血活性进行了测试。发现豚鼠红细胞最易被支原体裂解，而兔红细胞最不易被裂解。采用含有联苯胺和羊血的琼脂，建立了一种检测支原体菌株产生过氧化物的灵敏技术。用这种方法，过氧化物的产生与血琼脂上的溶血相关。过氧化物酶和过氧化氢酶抑制联苯胺反应和溶血。得出的结论是，所检测的支原体菌株的主要溶血素是一种过氧化物。
