通过与大肠杆菌接合使弗氏志贺菌菌株恢复毒力

FORMAL S B, LABREC E H, FALKOW S

J Bacteriol. 1965 Mar;89(3):835-8. doi: 10.1128/jb.89.3.835-838.1965.

Formal, Samuel B. (Walter Reed Army Institute of Research, Washington, D.C.), E. H. LaBrec, H. Schneider, and Stanley Falkow. Restoration of virulence to a strain of Shigella flexneri by mating with Escherichia coli. J. Bacteriol. 89:835-838. 1965.-Three spontaneous avirulent variants of Shigella flexneri 5 were isolated and employed as genetic recipients in matings with Escherichia coli K-12. The various hybrid classes isolated from these matings were subsequently examined for their ability to induce keratoconjunctivitis and to kill pretreated guinea pigs. All hybrids derived from two of the variants remained avirulent. A majority of the mal(+) hybrids of the third avirulent variant were observed to be restored to complete virulence.

福尔默尔，塞缪尔·B.（华盛顿特区沃尔特·里德陆军研究所），E.H.拉布雷克，H.施耐德，以及斯坦利·法尔科夫。通过与大肠杆菌接合使弗氏志贺菌菌株恢复毒力。《细菌学杂志》89:835 - 838。1965年。——分离出弗氏志贺菌5型的三个自发无毒变种，并将其用作与大肠杆菌K - 12接合时的遗传受体。随后检查从这些接合中分离出的各种杂交类群诱导角膜结膜炎和杀死预处理豚鼠的能力。来自两个变种的所有杂交种仍无毒性。观察到第三个无毒变种的大多数mal(+)杂交种恢复了完全毒力。