变形杆菌P18杆菌型与L型的脂质和脂多糖比较
COMPARISON OF LIPIDS AND LIPOPOLYSACCHARIDE FROM THE BACILLARY AND L FORMS OF PROTEUS P18.
作者信息
NESBITT J A, LENNARZ W J
出版信息
J Bacteriol. 1965 Apr;89(4):1020-5. doi: 10.1128/jb.89.4.1020-1025.1965.
Nesbitt, J. A., III (The Johns Hopkins University School of Medicine, Baltimore, Md.), and W. J. Lennarz. Comparison of lipids and lipopolysaccharides from the bacillary and L forms of Proteus P18. J. Bacteriol. 89:1020-1025. 1965.-Comparative studies on the L form of Proteus P18 and the parent bacterium grown in a defined medium showed that the L form contained 1.5 times as much extractable lipid (dry weight) as the bacillary form. The composition of the lipids extractable by chloroform-methanol was quite similar in the two bacterial forms. The occurrence of myristate and beta-hydroxymyristate in the bound, nonextractable lipid was found to be a reflection of the presence of lipopolysaccharide (LPS) in each organism. The bacillary organism contains three to four times as much LPS as the L form. The LPS isolated from both organisms contains heptose, hexosamine, phosphorus, 3-deoxyoctulosonate, glucose, galactose, and fatty acids.
内斯比特，J. A.，三世（约翰霍普金斯大学医学院，马里兰州巴尔的摩），以及W. J. 伦纳兹。变形杆菌P18杆菌型和L型的脂质与脂多糖比较。《细菌学杂志》89:1020 - 1025。1965年。——对在限定培养基中生长的变形杆菌P18的L型和亲本细菌进行的比较研究表明，L型所含可提取脂质（干重）是杆菌型的1.5倍。两种细菌形式中可被氯仿 - 甲醇提取的脂质组成相当相似。发现肉豆蔻酸和β - 羟基肉豆蔻酸在结合的、不可提取的脂质中的存在反映了每种生物体中脂多糖（LPS）的存在。杆菌型生物体所含的LPS是L型的三到四倍。从两种生物体中分离出的LPS均含有庚糖、己糖胺、磷、3 - 脱氧辛酮酸、葡萄糖、半乳糖和脂肪酸。
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