Redkar A A, Balsara B R, Bhisey A N, Sampat M B, Mittra I
Division of Laboratory Medicine, Tata Memorial Hospital, Bombay.
Indian J Med Res. 1992 Aug;96:223-9.
Flow cytometric estimation of DNA content (ploidy and S-phase fraction--SpF) was done on breast cancer tissues from 171 patients. Twenty eight per cent of the tumours were diploid and 72 per cent were aneuploid. SpF was measurable in 82 DNA histograms; of these 22.4 per cent had SpF less than 10 per cent, 34.1 per cent had SpF between 10-20 and 43.5 per cent had SpF greater than 20 per cent. The mean SpF of the measurable histograms was 19.01 per cent with a range 1.78 to 45.19 per cent. A significant correlation between DNA ploidy and SpF was observed (P less than 0.01). Eighty nine per cent of diploid tumours had SpF less than 10 per cent and 73 per cent of aneuploid tumours had SpF greater than 20 per cent. A significant correlation was also found between ploidy and SpF and oestrogen receptor (ER) status of the tumours (P less than 0.05) and between SpF and progesterone receptor (PgR) status of the tumours (P less than 0.05), but not between ploidy and PgR status of the tumours. A significant direct correlation was observed between SpF and tumour grade (P less than 0.05), but not between ploidy and tumour grade. No correlation was observed between DNA ploidy and SpF and tumour type, tumour size, axillary lymph node involvement, age and menopausal status of the patients. Although the incidence of breast cancer is one-third of that reported in the Western countries, there is apparently no biological difference between the various parameters studied.
对171例乳腺癌患者的组织进行了DNA含量（倍体和S期分数——SpF）的流式细胞术评估。28%的肿瘤为二倍体，72%为非整倍体。在82个DNA直方图中可测量SpF；其中22.4%的SpF小于10%，34.1%的SpF在10%-20%之间，43.5%的SpF大于20%。可测量直方图的平均SpF为19.01%，范围为1.78%至45.19%。观察到DNA倍体与SpF之间存在显著相关性（P<0.01）。89%的二倍体肿瘤SpF小于10%，73%的非整倍体肿瘤SpF大于20%。还发现倍体与SpF和肿瘤的雌激素受体（ER）状态之间存在显著相关性（P<0.05），以及SpF与肿瘤的孕激素受体（PgR）状态之间存在显著相关性（P<0.05），但倍体与肿瘤的PgR状态之间无相关性。观察到SpF与肿瘤分级之间存在显著正相关（P<0.05），但倍体与肿瘤分级之间无相关性。未观察到DNA倍体与SpF和肿瘤类型、肿瘤大小、腋窝淋巴结受累情况、患者年龄及绝经状态之间存在相关性。尽管乳腺癌的发病率是西方国家报道的三分之一，但在所研究的各种参数之间显然没有生物学差异。
