Ottestad L, Pettersen E O, Nesland J M, Hannisdal E, Fosså S D, Tveit K M
Department of Biochemistry, Norwegian Radium Hospital, Montebello, Oslo.
Pathol Res Pract. 1993 May;189(4):405-10. doi: 10.1016/S0344-0338(11)80327-4.
Fresh tumour tissue from 198 primary invasive breast carcinomas was analysed by DNA flow cytometry. 108 tumours were non-diploid. A significantly higher proportion of non-diploid tumours was found among node-positive patients, patients with oestrogen receptor negative tumours and among patients with ductal carcinomas. The survival of patients with diploid and non-diploid tumours was not significantly different (p = 0.1). Totally, 145 tumours were analyzed with respect to S-phase fraction (SPF). The distribution of SPF was different in diploid and non-diploid tumours. A low SPF group, defined as the lower SPF quartile (< or = 4.6% in diploid and < or = 8.5% in non-diploid tumours), was associated with highly differentiated tumours and oestrogen receptor positive tumours. Histological grading revealed a highly significant correlation to SPF. 57% of ductal carcinomas grade I (8 out of 14), 30% of ductal carcinomas grade II (20 out of 67) and 5% of ductal carcinomas grade III (2 out of 37) had a low SPF. Patients within the low SPF group had a significantly longer survival than had patients within the high SPF group (p = 0.006). In a multivariate analysis the SPF was found to be an additional prognostic factor next to node status and ER status.
对198例原发性浸润性乳腺癌的新鲜肿瘤组织进行了DNA流式细胞术分析。108个肿瘤为非二倍体。在淋巴结阳性患者、雌激素受体阴性肿瘤患者以及导管癌患者中，非二倍体肿瘤的比例显著更高。二倍体和非二倍体肿瘤患者的生存率无显著差异（p = 0.1）。总共对145个肿瘤进行了S期分数（SPF）分析。二倍体和非二倍体肿瘤的SPF分布不同。低SPF组定义为较低的SPF四分位数（二倍体肿瘤中≤4.6%，非二倍体肿瘤中≤8.5%），与高分化肿瘤和雌激素受体阳性肿瘤相关。组织学分级显示与SPF高度相关。I级导管癌的57%（14例中的8例）、II级导管癌的30%（67例中的20例）和III级导管癌的5%（37例中的2例）具有低SPF。低SPF组患者的生存期明显长于高SPF组患者（p = 0.006）。在多变量分析中，发现SPF是继淋巴结状态和雌激素受体状态之后的另一个预后因素。
