Wali A, Dhall K, Sanyal S N, Juneja R, Majumdar S
Department of Obstetrics & Gynaecology, Postgraduate Institute of Medical Education & Research, Chandigarh.
Indian J Med Res. 1992 Aug;96:261-5.
The protein component of human pulmonary surfactant was analysed. A purified surfactant preparation, after delipidation, was subjected to gel filtration on Sephadex G-200. The proteins present in the surfactant were categorised by means of SDS-polyacrylamide gel electrophoresis into serum and non-serum components. Molecular masses determination showed the presence of three sub-groups with molecular masses of 60-68 kDa, 28-36 kDa and 10-18 kDa, respectively. Antiserum generated against 28-36 kDa protein strongly reacted with the purified surfactant and amniotic fluid, while it did not show any cross reactivity with other groups of proteins and serum in a double diffusion immunoprecipitation assay. We propose that this protein is the major non-serum surfactant-associated protein present in human lung surfactant.
对人肺表面活性物质的蛋白质成分进行了分析。一种纯化的表面活性物质制剂在脱脂后，在Sephadex G - 200上进行凝胶过滤。通过SDS - 聚丙烯酰胺凝胶电泳将表面活性物质中存在的蛋白质分类为血清成分和非血清成分。分子量测定显示存在三个亚组，分子量分别为60 - 68 kDa、28 - 36 kDa和10 - 18 kDa。针对28 - 36 kDa蛋白质产生的抗血清与纯化的表面活性物质和羊水强烈反应，而在双向扩散免疫沉淀试验中，它与其他蛋白质组和血清没有任何交叉反应。我们认为这种蛋白质是存在于人肺表面活性物质中的主要非血清表面活性物质相关蛋白。
