Phelps D S, Taeusch H W, Benson B, Hawgood S
Biochim Biophys Acta. 1984 Dec 7;791(2):226-38. doi: 10.1016/0167-4838(84)90013-x.
We have prepared an antiserum against a serum-free extract of alveolar proteinosis lavage that recognizes the same proteins as an antiserum to human surfactant. Using one and two-dimensional gel electrophoresis, protein blotting and immunostaining we have found proteins with Mr of approx. 35 and 60 kDa to be present in every source of human surfactant we have examined. These proteins are immunologically related to those found in the lavage from alveolar proteinosis patients, have the same electrophoretic characteristics and are not found in serum. The 35 kDa protein is a group of at least eight isoforms ranging in relative molecular mass Mr from 32 to 36 kDa with isoelectric points between 4.8 and 5.5. Neuraminidase digestion studies have shown that at least part of this charge heterogeneity may be due to sialic acid residues. The less abundant form, with a Mr of about 60 kDa is also a sialoglycoprotein with similar isoelectric points.
我们制备了一种针对肺泡蛋白沉积症灌洗无血清提取物的抗血清，它能识别与抗人表面活性剂抗血清相同的蛋白质。通过一维及二维凝胶电泳、蛋白质印迹和免疫染色，我们发现在我们检测的每一种人表面活性剂来源中都存在分子量约为35 kDa和60 kDa的蛋白质。这些蛋白质与肺泡蛋白沉积症患者灌洗物中发现的蛋白质具有免疫相关性，具有相同的电泳特征，且在血清中未发现。35 kDa的蛋白质是一组至少八种同工型，相对分子质量Mr在32至36 kDa之间，等电点在4.8至5.5之间。神经氨酸酶消化研究表明，这种电荷异质性至少部分可能归因于唾液酸残基。含量较少的约60 kDa的形式也是一种具有相似等电点的唾液糖蛋白。
