Plishker G A, Gitelman H J
J Membr Biol. 1977 Aug 4;35(4):309-18. doi: 10.1007/BF01869956.
Intact human red blood cells incubated with ionophore A23187 and calcium develop a depletion of ATP that is dependent upon the concentrations of both A23187 and Ca. Incubations of fresh cells with 0.5 micrometer A23187 and concentrations of Ca at or below 70 micrometer produce a depletion of ATP without a net cellular uptake of Ca. In contrast, ATP-depleted cells display an ionophore-dependent cellular uptake of Ca, under identical conditions. A hypothesis is proposed that relates these ionophore-produced ATP depletions to active Ca extrusion by the Ca ATPase.
完整的人类红细胞与离子载体A23187和钙一起孵育时，会出现ATP耗竭，这取决于A23187和钙的浓度。将新鲜细胞与0.5微摩尔的A23187以及浓度为70微摩尔或更低的钙一起孵育，会导致ATP耗竭，而细胞对钙没有净摄取。相比之下，在相同条件下，ATP耗竭的细胞表现出离子载体依赖性的细胞对钙的摄取。提出了一个假设，将这些离子载体产生的ATP耗竭与钙ATP酶的主动钙外排联系起来。
