M13 DNA指纹图谱法：一种用于乳酸杆菌属分类和鉴定的新工具

M13 DNA fingerprinting, a new tool for classification and identification of Lactobacillus spp.

作者信息

Miteva V I, Abadjieva A N, Stefanova T T

机构信息

Institute of Microbiology, Bulgarian Academy of Sciences, Sofia.

出版信息

J Appl Bacteriol. 1992 Oct;73(4):349-54. doi: 10.1111/j.1365-2672.1992.tb04988.x.

DOI:10.1111/j.1365-2672.1992.tb04988.x

Abstract

The optimal conditions for the application of M13 DNA fingerprinting to the genus Lactobacillus were determined. Comparative fingerprint analysis of representative strains of Lactobacillus delbrueckii subsp. delbrueckii, Lact. delbrueckii subsp. lactis, Lact. delbrueckii subsp. bulgaricus, Lact. helveticus and Lact. casei permitted the differentiation of species, subspecies and individual strains and the quantitative determination of their genetic relatedness. The results confirm the high specificity of M13 DNA fingerprinting and indicate that it might be used in the classification of Lactobacillus spp.

摘要

确定了将M13 DNA指纹图谱应用于乳酸杆菌属的最佳条件。对德氏乳杆菌德氏亚种、德氏乳杆菌乳酸亚种、德氏乳杆菌保加利亚亚种、瑞士乳杆菌和干酪乳杆菌的代表性菌株进行比较指纹分析，能够区分物种、亚种和单个菌株，并定量测定它们的遗传相关性。结果证实了M13 DNA指纹图谱具有高度特异性，并表明它可用于乳酸杆菌属的分类。

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