注意：通过扩增核糖体DNA限制性分析鉴定德氏乳杆菌保加利亚亚种和乳酸亚种的乳制品分离株。
Note: identification of Lactobacillus delbrueckii subspecies bulgaricus and subspecies lactis dairy isolates by amplified rDNA restriction analysis.
作者信息
Giraffa G, De Vecchi P, Rossetti L
机构信息
Istituto Sperimentale Lattiero Caseario, Lodi, Italy.
出版信息
J Appl Microbiol. 1998 Nov;85(5):918-24. doi: 10.1046/j.1365-2672.1998.00606.x.
Amplification and restriction analysis of the 16S rRNA gene (PCR-ARDRA) was used to identify Lactobacillus delbrueckii dairy isolates and to assign them to its three subspecies (delbrueckii, lactis and bulgaricus). PCR-ARDRA allowed confirmation of predictions from the DNA database regarding the restriction mapping of the 16S sequence to be verified in practice. PCR-ARDRA was shown to be a reliable and rapid method for identifying Lact. delbrueckii isolates at the subspecies level and for differentiating this species from Lact. helveticus and Lact. acidophilus.
利用16S rRNA基因的扩增与限制性分析（PCR-ARDRA）来鉴定德氏乳杆菌的乳制品分离株，并将其归为三个亚种（德氏亚种、乳酸亚种和保加利亚亚种）。PCR-ARDRA可以证实DNA数据库中关于16S序列限制性图谱的预测在实际中得到验证。结果表明，PCR-ARDRA是一种可靠且快速的方法，可用于在亚种水平上鉴定德氏乳杆菌分离株，并将该物种与瑞士乳杆菌和嗜酸乳杆菌区分开来。
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