Zhong W, Millsap K, Bialkowska-Hobrzanska H, Reid G
Lawson Research Institute, University of Western Ontario, London, Ontario, Canada.
Appl Environ Microbiol. 1998 Jul 1;64(7):2418-23. doi: 10.1128/AEM.64.7.2418-2423.1998.
A total of 64 type, reference, clinical, health food, and stock isolates of microaerophilic Lactobacillus species were examined by restriction fragment length polymorphisms. Of particular interest were members of six of the eight species most commonly recovered from the vaginas of healthy premenopausal women, namely, Lactobacillus jensenii, L. casei, L. rhamnosus, L. acidophilus, L. plantarum, and L. fermentum. Six main groupings were identified on the basis of ribotyping. This technique was able to classify fresh isolates to the species level. In the case of the ribotype A grouping for L. rhamnosus, differences between strains were evident by chromosome typing (chromotyping). Many isolates did not possess plasmids. Six L. rhamnosus strains isolated from four different health food products appeared to be identical to L. rhamnosus ATCC 21052. The molecular typing system is useful for identifying and differentiating Lactobacillus isolates. Studies of strains of potential importance to the urogenital flora should include molecular characterization as a means of comparing genetic traits with those of strains whose characteristics associated with colonization and antagonism against pathogens have been defined.
采用限制性片段长度多态性方法对总共64株微需氧乳杆菌属的标准菌株、参考菌株、临床菌株、保健食品菌株和库存菌株进行了检测。特别值得关注的是从健康绝经前女性阴道中最常分离出的8个物种中的6个物种的成员，即詹氏乳杆菌、干酪乳杆菌、鼠李糖乳杆菌、嗜酸乳杆菌、植物乳杆菌和发酵乳杆菌。根据核糖体分型确定了6个主要分组。该技术能够将新鲜分离株分类到物种水平。就鼠李糖乳杆菌的核糖体A组而言，通过染色体分型（染色体组型分析）可明显看出菌株之间的差异。许多分离株不具有质粒。从4种不同保健食品中分离出的6株鼠李糖乳杆菌菌株似乎与鼠李糖乳杆菌ATCC 21052相同。该分子分型系统有助于鉴定和区分乳杆菌分离株。对泌尿生殖系统菌群具有潜在重要性的菌株研究应包括分子特征分析，以此作为将遗传特性与那些已明确其与定植及对病原体拮抗作用相关特征的菌株进行比较的一种手段。
