甘氨酸甜菜碱和钾离子是极端嗜盐产甲烷菌嗜盐嗜甲烷菌Z7302中的主要相容性溶质。
Glycine betaine and potassium ion are the major compatible solutes in the extremely halophilic methanogen Methanohalophilus strain Z7302.
作者信息
Lai M C, Gunsalus R P
机构信息
Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90024.
出版信息
J Bacteriol. 1992 Nov;174(22):7474-7. doi: 10.1128/jb.174.22.7474-7477.1992.
Abstract
Methanohalophilus strain Z7302 was previously isolated from a hypersaline environment and grows over a range of NaCl concentrations from 1.7 to 4.4 M. We examined the relationships between cell growth rate, cell volume, and intracellular solute concentrations with increasing salinity. This extremely halophilic methanogen synthesized three zwitterionic compounds, beta-glutamine, N epsilon-acetyl-beta-lysine, and glycine betaine, and also accumulated potassium ion as compatible solutes to balance the external and internal osmotic pressures. Potassium and glycine betaine were the predominant compatible solutes when Methanohalophilus strain Z7302 was grown at high external NaCl concentrations and approached intracellular levels of 3 and 4 M, respectively.
摘要
嗜盐嗜甲烷菌Z7302菌株先前是从高盐环境中分离出来的，能在1.7至4.4 M的一系列氯化钠浓度范围内生长。我们研究了随着盐度增加，细胞生长速率、细胞体积和细胞内溶质浓度之间的关系。这种极端嗜盐产甲烷菌合成了三种两性离子化合物，β-谷氨酰胺、Nε-乙酰-β-赖氨酸和甘氨酸甜菜碱，并且还积累钾离子作为相容性溶质来平衡外部和内部渗透压。当嗜盐嗜甲烷菌Z7302菌株在高外部氯化钠浓度下生长时，钾和甘氨酸甜菜碱分别是主要的相容性溶质，且细胞内水平分别接近3 M和4 M。
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