Sillau A H, Banchero N
Pflugers Arch. 1977 Jul 19;369(3):269-71. doi: 10.1007/BF00582194.
A simple and reliable technique for the visualization of capillaries in skeletal muscel of dogs, guinea pigs and rats is decribed. 10--20 micrometer frozen sections were incubated in a medium containing Ca2+ and ATP following acid preincubation (pH 3.8--4.2). The capillaries stained in black and were readily seen surrounding the muscle fibers. Serial sections were also treated for alkaline phosphatase. Values for capillary density using both methods were not different.
描述了一种用于观察狗、豚鼠和大鼠骨骼肌中毛细血管的简单可靠技术。在酸预孵育（pH 3.8 - 4.2）后，将10 - 20微米的冰冻切片在含有Ca2+和ATP的培养基中孵育。毛细血管染成黑色，很容易在肌纤维周围看到。连续切片也进行了碱性磷酸酶处理。使用这两种方法得到的毛细血管密度值没有差异。
