Yamaguchi A, Maeda J, Okumoto T, Katsuta S
Department of General Education, Health Sciences University of Hokkaido, Japan.
Eur J Appl Physiol Occup Physiol. 1994;69(5):387-91. doi: 10.1007/BF00865400.
This investigation was undertaken to determine whether a severe limitation of blood supply by external iliac artery ligation can change the number and density of capillaries in rat soleus muscle. The external iliac artery in one hindlimb was ligated for 2, 7 or 28 days, and the other, sham-operated, hindlimb was used as a control. Muscle blood flow in the ischaemic soleus muscle at 2, 7 and 28 days after external iliac artery ligation was significantly decreased compared with the control. The muscle fibre area and the ratio of the fibre area to body mass in the ischaemic soleus muscle at 28 days after the external iliac artery ligation were significantly reduced in the ischaemic soleus muscle, but no change in the number of capillaries per fibre was observed. Capillary density per millimetres squared at 28 days and the ratios of the number of capillaries around type I fibres to the fibre area at 7 and 28 days were significantly increased in the ischaemic soleus muscle (P < 0.05). These results suggested that long-term severe limitation of blood flow in the soleus muscle by ligation of the external iliac artery could have induced the increase in capillary density, as a result of atrophy of muscle fibres rather than capillary growth.
本研究旨在确定通过结扎髂外动脉造成的严重血液供应受限是否会改变大鼠比目鱼肌中毛细血管的数量和密度。将一侧后肢的髂外动脉结扎2、7或28天，另一侧后肢进行假手术作为对照。与对照组相比，髂外动脉结扎后2、7和28天时，缺血比目鱼肌的肌肉血流量显著降低。髂外动脉结扎28天后，缺血比目鱼肌的肌纤维面积以及肌纤维面积与体重的比值显著降低，但每根纤维的毛细血管数量未观察到变化。缺血比目鱼肌在28天时每平方毫米的毛细血管密度以及在7天和28天时I型纤维周围毛细血管数量与纤维面积的比值均显著增加（P < 0.05）。这些结果表明，通过结扎髂外动脉对比目鱼肌进行长期严重的血流限制可能会由于肌纤维萎缩而非毛细血管生长导致毛细血管密度增加。
