STOJCESKI T K, MALPAS J S, WITTS L J
J Clin Pathol. 1965 Jul;18(4):446-52. doi: 10.1136/jcp.18.4.446.
The total iron-binding capacity of serum has been estimated using a colorimetric method, a radioisotopic technique, and a method employing immunodiffusion. The modified isotopic technique described was considered to be the method of choice. The total iron-binding capacity starts to increase at an early stage of an iron-deficiency anaemia. It is decreased in infectious and malignant disease. Close correlation exists between the level of saturation of the total iron-binding capacity and the haemoglobin level in patients with uncomplicated iron-deficiency anaemia. In patients with disease of the liver a difference was sometimes found between the total iron-binding capacity determined by the immunodiffusion method and by the two other techniques, the values with the immunodiffusion method being distinctly lower. This suggests the presence in the serum of iron which is not bound to transferrin. It is possible that ferritin was present in the serum in these cases.
血清总铁结合力已采用比色法、放射性同位素技术以及免疫扩散法进行评估。所描述的改良同位素技术被认为是首选方法。血清总铁结合力在缺铁性贫血的早期阶段开始升高。在感染性疾病和恶性疾病中其降低。在无并发症的缺铁性贫血患者中，总铁结合力的饱和度水平与血红蛋白水平之间存在密切相关性。在肝脏疾病患者中，有时会发现免疫扩散法与其他两种技术所测定的血清总铁结合力存在差异，免疫扩散法测定的值明显更低。这表明血清中存在未与转铁蛋白结合的铁。在这些病例中血清中可能存在铁蛋白。
