Webley G E, Knight P G, Hearn J P
MRC/AFRC Comparative Physiology Group, Institute of Zoology, London, UK.
J Reprod Fertil. 1992 Sep;96(1):385-93. doi: 10.1530/jrf.0.0960385.
The role of the embryo in promoting increased plasma concentrations of immunoreactive inhibin after conception in the marmoset monkey was determined by flushing embryos from the uterus between days 5 and 9 after ovulation (implantation commences on days 11-12). Blood samples were taken from each animal (three times a week) after ovulation until the end of the luteal phase. Plasma inhibin concentrations were measured using a radioimmunoassay based on antisera against a synthetic fragment of the alpha-subunit of human inhibin. When embryos were flushed on days 5 and 6 (n = 6) after ovulation inhibin concentrations did not exceed 250 ng ml-1 for the duration of the luteal phase. In contrast when embryos were flushed on days 7 (n = 4), 8 (n = 4) and 9 (n = 3) maximum concentrations of inhibin always exceeded 250 ng ml-1, reaching > 400 ng ml-1 when embryos were flushed on days 8 and 9. Inhibin concentrations remained high for the duration of the luteal phase, which varied in length between 20 and 32 days. Significantly (P < 0.01) higher mean plasma concentrations of immunoreactive inhibin were first recorded on days 7-8 after ovulation in animals that had embryos flushed on days 7, 8 and 9 compared with concentrations in animals that had embryos flushed on days 5 and 6. Inhibin could not be detected in the medium of embryos cultured for up to 2 weeks.(ABSTRACT TRUNCATED AT 250 WORDS)
通过在排卵后第5至9天从子宫冲洗胚胎（着床在第11 - 12天开始），确定了狨猴受孕后胚胎在促进免疫反应性抑制素血浆浓度升高方面的作用。排卵后，每周三次从每只动物采集血样，直至黄体期结束。使用基于抗人抑制素α亚基合成片段抗血清的放射免疫分析法测量血浆抑制素浓度。当在排卵后第5天和第6天（n = 6）冲洗胚胎时，黄体期内抑制素浓度不超过250 ng/ml。相比之下，当在第7天（n = 4）、第8天（n = 4）和第9天（n = 3）冲洗胚胎时，抑制素的最高浓度总是超过250 ng/ml，在第8天和第9天冲洗胚胎时达到>400 ng/ml。抑制素浓度在黄体期持续保持高水平，黄体期长度在20至32天之间变化。与在第5天和第6天冲洗胚胎的动物相比，在第7、8和9天冲洗胚胎的动物中，排卵后第7 - 8天首次记录到免疫反应性抑制素的平均血浆浓度显著更高（P < 0.01）。在培养长达2周的胚胎培养基中未检测到抑制素。（摘要截短至250字）
