不同激素条件对大鼠肝脏烟酰胺核苷酸浓度及氧化还原状态的影响

THE EFFECT OF DIFFERENT HORMONAL CONDITIONS ON THE CONCENTRATION AND OXIDOREDUCTION STATE OF THE NICOTINAMIDE NUCLEOTIDES OF RAT LIVER.

作者信息

GREENBAUM A L, CLARK J B

出版信息

Biochem J. 1965 Apr;95(1):167-79. doi: 10.1042/bj0950167.

DOI:10.1042/bj0950167

PMID:14333553

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1215191/

Abstract

The concentration and oxidoreduction state of the liver nicotinamide nucleotides of rats subjected to a number of hormonal treatments have been measured. 2. Adrenalectomy decreases the NADP(+) content by 80% but has little effect on NAD(+), NADH or NADPH. High doses of cortisone produce similar changes, but more physiological doses (5mug. daily) tend to increase the NADP(+) content. 3. Glucagon treatment of normal rats lowered the NADH and NADP(+) concentrations but did not affect the total amounts present. Growth hormone increased the concentrations and total amounts of NAD(+) and NADH but significantly decreased the concentrations and total amounts of NADP(+) and NADPH. 4. Measurements have been made of a number of enzymes in the livers of adrenalectomized and glucagon-treated rats that could affect the oxidoreduction state of NADP. The activities of glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase are not affected by adrenalectomy or treatment with cortisone or glucagon. Nor does adrenalectomy affect the activity of NADPH-cytochrome c oxidoreductase or NADPH-glutathione oxidoreductase. The hepatic content of glutathione is, however, decreased 50% by adrenalectomy. 5. Measurements of the oxidation of [1-(14)C]glucose and [6-(14)C]glucose by liver slices from adrenalectomized rats showed that glucose oxidation was substantially normal, although phenazine methosulphate caused a smaller stimulation of the oxidation of C-1 of [1-(14)C]glucose in slices from the livers of adrenalectomized rats than it did with slices from controls. The hepatic synthesis of lipids from [1-(14)C]glucose was marginally increased in adrenalectomized rats. 6. The additional NADP(+) found when liver is extracted with 0.02n-sulphuric acid-0.1m-sodium sulphate is less affected than the NADP(+) extracted with 0.1n-hydrochloric acid in adrenalectomized or glucagon-treated rats. Hooded Norway rats appear to have less of this extra form of NADP(+) than albino rats. 7. An attempt has been made to correlate the observed changes in the nicotinamide nucleotides with metabolic patterns prevailing in different hormonal conditions.

摘要

已对接受多种激素处理的大鼠肝脏烟酰胺核苷酸的浓度和氧化还原状态进行了测定。2. 肾上腺切除术使NADP(+)含量降低80%，但对NAD(+)、NADH或NADPH影响很小。高剂量的可的松产生类似变化，但更生理剂量（每日5微克）倾向于增加NADP(+)含量。3. 用胰高血糖素处理正常大鼠可降低NADH和NADP(+)浓度，但不影响其总量。生长激素增加了NAD(+)和NADH的浓度及总量，但显著降低了NADP(+)和NADPH的浓度及总量。4. 已对肾上腺切除和胰高血糖素处理的大鼠肝脏中一些可能影响NADP氧化还原状态的酶进行了测定。6-磷酸葡萄糖脱氢酶和6-磷酸葡萄糖酸脱氢酶的活性不受肾上腺切除术或可的松或胰高血糖素处理的影响。肾上腺切除术也不影响NADPH-细胞色素c氧化还原酶或NADPH-谷胱甘肽氧化还原酶的活性。然而，肾上腺切除术使肝脏中谷胱甘肽含量降低50%。5. 对肾上腺切除大鼠肝脏切片中[1-(14)C]葡萄糖和[6-(14)C]葡萄糖的氧化测定表明，葡萄糖氧化基本正常，尽管硫酸吩嗪甲酯对肾上腺切除大鼠肝脏切片中[1-(14)C]葡萄糖C-1氧化的刺激作用比对对照肝脏切片的刺激作用小。肾上腺切除大鼠肝脏中由[1-(14)C]葡萄糖合成脂质略有增加。6. 当用0.02N硫酸-0.1M硫酸钠提取肝脏时发现的额外NADP(+)，在肾上腺切除或胰高血糖素处理的大鼠中，其受影响程度小于用0.1N盐酸提取的NADP(+)。挪威黑帽大鼠这种额外形式的NADP(+)似乎比白化大鼠少。7. 已尝试将观察到的烟酰胺核苷酸变化与不同激素条件下的代谢模式联系起来。