Agricultural Research Council Unit of Developmental Botany, 181A Huntingdon Road, CB3 ODY, Cambridge, UK.
Planta. 1974 Sep;117(3):251-8. doi: 10.1007/BF00388398.
Broad-bean cotyledons lost about 80% of their RNA during the first 30 days of seed germination. Both high-molecular-weight ribosomal (r) RNA and low-molecular-weight RNA were degraded at about the same rate. Only small quantities of breakdown products of rRNA were detected within senescent tissue. This finding contrasts strongly with the very rapid cleavage of rRNA and the accumulation of breakdown products when the same cotyledons were homogenised and then incubated at 25°. It would appear, therefore, that there was a barrier between ribonuclease and most ribosomes in vivo which was disrupted by homogenisation. The results are discussed in relation to the mechanism of ribosome katabolism during senescence.
在种子发芽的头 30 天里，宽叶豆的子叶大约损失了 80%的 RNA。高分子量核糖体 (r) RNA 和低分子量 RNA 的降解速度大致相同。在衰老组织中只检测到 rRNA 降解产物的少量。这一发现与以下事实形成鲜明对比：当相同的子叶被匀浆化然后在 25°C 下孵育时，rRNA 非常迅速地切割，并且降解产物积累。因此，似乎在体内存在一种阻止核糖核酸酶与大多数核糖体相互作用的屏障，这种屏障在匀浆化时被打破。结果与衰老过程中核糖体分解代谢的机制有关。
