Fournet A, Angelo A, Muñoz V, Roblot F, Hocquemiller R, Cavé A
Institut Français de Recherche Scientifique pour le Développement en Coopération (ORSTOM), Département Santé, Paris, France.
J Ethnopharmacol. 1992 Sep;37(2):159-64. doi: 10.1016/0378-8741(92)90074-2.
The stem barks of Pera benensis are employed by the Chimane Indians in the Bolivian Amazonia as treatment of cutaneous leishmaniasis caused by the protozoan Leishmania braziliensis. The chloroform extracts containing quinones were found active against the promastigote forms of Leishmania and the epimastigote forms of Trypanosoma cruzi at 10 micrograms ml-1. The activity guided fractionation of the extract by chromatography afforded active compounds. Their structures were elucidated, by spectral and chemical studies, as known naphthoquinones, plumbagin, 3,3'-biplumbagin, 8-8'-biplumbagin, and triterpene, lupeol. The activity in vitro of each compound was evaluated against 5 strains of Leishmania (promastigote), 6 strains of Trypanosoma cruzi (epimastigote) and the intracellular form (amastigote) of Leishmania amazonensis. The baseline drugs used were Glucantime and pentamidine (Leishmania spp.), nifurtimox and benznidazole (T. cruzi). Plumbagin was the most active compound in vitro. This study has demonstrated that Pera benensis, a medicinal plant used in folk medicine, is an efficient treatment of cutaneous leishmaniasis.
玻利维亚亚马逊地区的奇曼内印第安人使用贝氏佩拉树的茎皮来治疗由原生动物巴西利什曼原虫引起的皮肤利什曼病。发现含有醌类的氯仿提取物在10微克/毫升的浓度下对利什曼原虫的前鞭毛体形式和克氏锥虫的上鞭毛体形式具有活性。通过色谱法对提取物进行活性导向分级分离得到了活性化合物。通过光谱和化学研究阐明了它们的结构，为已知的萘醌类化合物、白花丹素、3,3'-联白花丹素、8-8'-联白花丹素和三萜类化合物羽扇豆醇。评估了每种化合物对5株利什曼原虫（前鞭毛体）、6株克氏锥虫（上鞭毛体）以及亚马逊利什曼原虫的细胞内形式（无鞭毛体）的体外活性。使用的对照药物是葡糖胺锑钠和喷他脒（用于利什曼原虫属）、硝呋替莫和苯硝唑（用于克氏锥虫）。白花丹素是体外活性最强的化合物。这项研究表明，民间医学中使用的药用植物贝氏佩拉树是治疗皮肤利什曼病的有效药物。
