Moein Mahmoodreza, Hatam Gholamreza, Taghavi-Moghadam Razieh, Zarshenas Mohammad M
Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Pharmacognosy, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.
J Evid Based Complementary Altern Med. 2017 Jan;22(1):31-36. doi: 10.1177/2156587215623435. Epub 2016 Jan 8.
Petroleum ether, chloroform, ethyl acetate, and n-butanol fractions of Greek juniper (Juniperus excelsa M.Bieb. from the family Cupressaceae) were evaluated for antileishmanial activities against Leishmania major promastigotes compared to meglumine antimoniate (Glucantime). In vitro toxicity assay was performed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide and microplate ELISA reader. Extracts were prepared in ethanol/dimethyl sulfoxide (80/20) at 10 to 0.62 mg/mL. The standard was prepared in phosphate-buffered saline at 500 to 15.62 mg/mL. Both leaf and fruit extracts and related fractions showed strong inhibitory effects against promastigotes, significantly different from that of the standard. The leaf extract and the respective petroleum ether fraction showed maximum effectiveness compared to other fractions and also fruit extract and fractions (IC = 1.89 ± 0.03 and 0.90 ± 0.03 mg/mL, respectively). Regarding the potent activities of nonpolar fractions of Greek juniper leaf extract, these fractions can be suggested for further investigation.
将希腊桧（柏科的刺叶桧）的石油醚、氯仿、乙酸乙酯和正丁醇馏分与葡甲胺锑酸盐（葡糖胺锑）相比，评估其对硕大利什曼原虫前鞭毛体的抗利什曼活性。使用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四氮唑和酶标仪进行体外毒性试验。提取物用乙醇/二甲基亚砜（80/20）配制成10至0.62毫克/毫升。标准品用磷酸盐缓冲盐水配制成500至15.62毫克/毫升。叶提取物和果实提取物及其相关馏分对前鞭毛体均表现出强烈的抑制作用，与标准品有显著差异。与其他馏分以及果实提取物和馏分相比，叶提取物及其相应的石油醚馏分显示出最大的效果（IC50分别为1.89±0.03和0.90±0.03毫克/毫升）。鉴于希腊桧叶提取物非极性馏分的强效活性，可建议对这些馏分进行进一步研究。
