Carrillo M C, Kanai S, Sato Y, Kitani K
Department of Clinical Physiology, Tokyo Metropolitan Institute of Gerontology, Japan.
Mech Ageing Dev. 1992 Sep;65(2-3):187-98. doi: 10.1016/0047-6374(92)90035-c.
Enzyme activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) were determined in the liver as well as several specific brain regions of young and old Fischer-344 rats of both sexes. In the liver of male rats, activities of CAT as well as Mn-SOD were lower, while activities of Cu Zn-SOD were higher in old (30-month-old) rats than in young (7-month-old) ones. Activities of total SOD as well as GSH Px were comparable for young and old male rat livers. In contrast to male rats, in female rat livers, activities of CAT were significantly higher in old (28-months-old) rats, while activities of Mn-SOD were slightly (but significantly) higher in old rat livers. In old male rats, activities of Mn-SOD were significantly higher than in young males in several specific regions of the brain (the substantia nigra (s. nigra), striatum, hippocampus) but lower in the cerebellum. In particular, SOD activities in s. nigra, striatum and hippocampus in old male rats were several fold higher than corresponding values in young male rats. Activities of Cu Zn-SOD were generally unchanged with age. Activities of CAT as well as GSH-Px (both Se-dependent and non-Se-dependent forms) were also relatively unaffected by age. In female rat brains, activities of Mn-SOD as well as those of others all remained mostly unaffected by aging, although there was a general tendency of slightly higher activities in most cerebral regions for Mn-SOD in old female rats. Thus, age-related changes of these antioxidant enzymes in the liver and brain are markedly sex dependent and some enzyme activities (such as CAT in the liver) change in an opposite direction with age. Changes of Mn-SOD in the brain were markedly region-specific in male rats. Results suggest that the significance of the changes of these antioxidant enzyme activities during aging needs to be carefully interpreted, taking into consideration the fact that changes are markedly variable depending on sex as well as the organs and brain regions examined.
在雄性和雌性年轻及老年Fischer-344大鼠的肝脏以及几个特定脑区中测定了超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）的酶活性。在雄性大鼠肝脏中，老年（30月龄）大鼠的CAT以及锰超氧化物歧化酶（Mn-SOD）活性较低，而铜锌超氧化物歧化酶（Cu Zn-SOD）活性高于年轻（7月龄）大鼠。年轻和老年雄性大鼠肝脏中的总SOD以及谷胱甘肽过氧化物酶（GSH Px）活性相当。与雄性大鼠不同，在雌性大鼠肝脏中，老年（28月龄）大鼠的CAT活性显著更高，而老年大鼠肝脏中的Mn-SOD活性略高（但显著）。在老年雄性大鼠中，Mn-SOD活性在几个特定脑区（黑质、纹状体、海马体）显著高于年轻雄性大鼠，但在小脑中较低。特别是，老年雄性大鼠黑质、纹状体和海马体中的SOD活性比年轻雄性大鼠的相应值高几倍。Cu Zn-SOD活性一般不随年龄变化。CAT以及GSH-Px（硒依赖型和非硒依赖型）的活性也相对不受年龄影响。在雌性大鼠脑中，Mn-SOD以及其他酶的活性大多不受衰老影响，尽管老年雌性大鼠大多数脑区中Mn-SOD活性有略高的总体趋势。因此，肝脏和脑中这些抗氧化酶的年龄相关变化明显具有性别依赖性，一些酶活性（如肝脏中的CAT）随年龄呈相反方向变化。雄性大鼠脑中Mn-SOD的变化明显具有区域特异性。结果表明，在解释衰老过程中这些抗氧化酶活性变化的意义时需要谨慎，要考虑到这些变化因性别以及所检查的器官和脑区而显著不同这一事实。
