Pan J T, Li C S, Tang K C, Lin J Y
Institute of Physiology, National Yang-Ming Medical College, Taipei, Taiwan, ROC.
Neurosci Lett. 1992 Sep 14;144(1-2):157-60. doi: 10.1016/0304-3940(92)90739-t.
Extracellular single-unit recording was conducted in hypothalamic arcuate (ARC) and suprachiasmatic nucleus (SCN) in rat brain slices. The perifusion medium was switched from normal artificial cerebrospinal fluid (ACSF) to ACSF containing low Ca2+ and high Mg2+ to test the effect of extracellular calcium on the spontaneous activities of these neurons and their responses to some test agents. It was found that almost all of the ARC and SCN units tested increased their firing rates in low Ca2+, high Mg2+ ACSF, and exhibited more significant responses to excitatory test agents. The effects of low Ca2+, high Mg2+ ACSF were repeatable and reversible. The results suggest that extracellular Ca2+ is essential in maintaining the membrane stability of most hypothalamic neurons and most agents tested are acting postsynaptically on the unit recorded.
在大鼠脑片中，对下丘脑弓状核（ARC）和视交叉上核（SCN）进行了细胞外单单位记录。将灌流介质从正常人工脑脊液（ACSF）切换为含有低钙和高镁的ACSF，以测试细胞外钙对这些神经元自发活动及其对某些测试剂反应的影响。结果发现，几乎所有测试的ARC和SCN单位在低钙、高镁ACSF中放电率增加，并对兴奋性测试剂表现出更明显的反应。低钙、高镁ACSF的作用是可重复且可逆的。结果表明，细胞外钙对于维持大多数下丘脑神经元的膜稳定性至关重要，并且大多数测试剂是在突触后作用于所记录的单位。
