Herbison A E, Hubbard J I, Sirett N E
Brain Res. 1986 Feb 5;364(2):391-5. doi: 10.1016/0006-8993(86)90854-1.
The effects of neurotensin (NT) on 41 spontaneously active neurons in the rat hypothalamic arcuate nucleus (ARC) were determined using an in vitro brain slice technique. Addition of NT (1 microM to 10 nM) to the perifusing medium allowed the action of known concentrations of NT to be examined. Concentrations of peptide greater than 10 nM evoked excessive depolarization of sensitive neurons resulting in long periods of postexcitatory inhibition. At 10 nM, NT evoked reversible and repeatable excitatory (20%) and inhibitory (25%) responses. When synaptic activity was blocked by lowering the Ca2+ and raising the Mg2+ concentration of the medium only excitatory responses (25% of units tested) were observed. These results demonstrate a potent excitatory action of NT within the hypothalamic ARC at the single cell level.
采用体外脑片技术，测定了神经降压素（NT）对大鼠下丘脑弓状核（ARC）中41个自发活动神经元的影响。向灌流培养基中添加NT（1微摩尔至10纳摩尔），以便研究已知浓度的NT的作用。大于10纳摩尔的肽浓度会引起敏感神经元过度去极化，导致长时间的兴奋后抑制。在10纳摩尔时，NT引起可逆且可重复的兴奋反应（20%）和抑制反应（25%）。当通过降低培养基中的Ca2+浓度和提高Mg2+浓度来阻断突触活动时，仅观察到兴奋反应（测试单元的25%）。这些结果证明了NT在下丘脑ARC内单细胞水平上具有强大的兴奋作用。
