利用荧光素标记抗体对雪貂流感感染的研究。II. 可溶性抗原在核荧光和交叉反应中的作用。
Studies on influenza infection in ferrets by means of fluorescein-labelled antibody. II. The role of soluble antigen in nuclear fluorescence and cross-reactions.
作者信息
LIU C
出版信息
J Exp Med. 1955 Jun 1;101(6):677-86. doi: 10.1084/jem.101.6.677.
Abstract
Yellow-green fluorescence representing viral antigens was detected in both the nucleus and cytoplasm of epithelial cells of the respiratory tract in ferrets infected with influenza virus. This nuclear fluorescence was the chief manifestation of cross-fluorescent staining reactions among three strains of influenza A virus studied, PR8, Farrington, and Fm(1). Absorption experiments with influenza viral V and soluble S antigens showed that S antigen was responsible for the presence of fluorescence in the nucleus and for the cross-staining reactions among these strains.
摘要
在感染流感病毒的雪貂呼吸道上皮细胞的细胞核和细胞质中均检测到代表病毒抗原的黄绿色荧光。这种核荧光是所研究的三种甲型流感病毒株（PR8、Farrington和Fm(1)）之间交叉荧光染色反应的主要表现。用流感病毒V抗原和可溶性S抗原进行的吸收实验表明，S抗原是细胞核中荧光存在以及这些病毒株之间交叉染色反应的原因。
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