• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

在细胞核中可检测到由猿猴病毒40载体中的克隆DNA合成的流感病毒核蛋白。

The influenza virus nucleoprotein synthesized from cloned DNA in a simian virus 40 vector is detected in the nucleus.

作者信息

Lin B C, Lai C J

出版信息

J Virol. 1983 Jan;45(1):434-8. doi: 10.1128/JVI.45.1.434-438.1983.

DOI:10.1128/JVI.45.1.434-438.1983
PMID:6296449
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC256425/
Abstract

We obtained DNA sequences coding for the nucleoprotein (NP) of an influenza A virus by reverse transcription of virion RNA with synthetic oligonucleotide primers. Terminal sequence analysis showed that the cloned gene contained a full-length copy of the virion RNA segment. The NP-specific DNA was inserted into the late region of a simian virus 40 vector, and the DNA recombinant was propagated in the presence of an early simian virus 40 temperature-sensitive mutant helper. Infection of African green monkey kidney cells with the recombinant produced a polypeptide immunoprecipitable with NP-specific antisera. The polypeptide product had a molecular weight of 56,000, identical to that of the nucleoprotein of influenza virus as estimated on polyacrylamide gels. The putative NP was detected in the nucleus of infected primate cells by an immunofluorescence assay. This nuclear localization of NP from recombinant DNA was similar to that seen during influenza virus infection.

摘要

我们使用合成寡核苷酸引物对病毒粒子RNA进行逆转录，从而获得了编码甲型流感病毒核蛋白（NP）的DNA序列。末端序列分析表明，克隆基因包含病毒粒子RNA片段的全长拷贝。将NP特异性DNA插入猿猴病毒40载体的晚期区域，并在早期猿猴病毒40温度敏感突变体辅助病毒存在的情况下繁殖DNA重组体。用该重组体感染非洲绿猴肾细胞，产生了一种可被NP特异性抗血清免疫沉淀的多肽。该多肽产物的分子量为56,000，与在聚丙烯酰胺凝胶上估计的流感病毒核蛋白分子量相同。通过免疫荧光测定法在受感染的灵长类细胞的细胞核中检测到推定的NP。来自重组DNA的NP的这种核定位与流感病毒感染期间观察到的情况相似。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3698/256425/2b5c401dc51c/jvirol00148-0453-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3698/256425/d7e23045a635/jvirol00148-0452-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3698/256425/2b5c401dc51c/jvirol00148-0453-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3698/256425/d7e23045a635/jvirol00148-0452-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3698/256425/2b5c401dc51c/jvirol00148-0453-a.jpg

相似文献

1
The influenza virus nucleoprotein synthesized from cloned DNA in a simian virus 40 vector is detected in the nucleus.在细胞核中可检测到由猿猴病毒40载体中的克隆DNA合成的流感病毒核蛋白。
J Virol. 1983 Jan;45(1):434-8. doi: 10.1128/JVI.45.1.434-438.1983.
2
Functional expression in primate cells of cloned DNA coding for the hemagglutinin surface glycoprotein of influenza virus.编码流感病毒血凝素表面糖蛋白的克隆DNA在灵长类细胞中的功能性表达。
Proc Natl Acad Sci U S A. 1981 Sep;78(9):5488-92. doi: 10.1073/pnas.78.9.5488.
3
Functional expression of influenza A viral nucleoprotein in cells transformed with cloned DNA.甲型流感病毒核蛋白在经克隆DNA转化的细胞中的功能性表达。
Virology. 1986 Oct 15;154(1):144-54. doi: 10.1016/0042-6822(86)90437-x.
4
Oriented synthesis and cloning of influenza virus nucleoprotein cDNA that leads to its expression in mammalian cells.流感病毒核蛋白cDNA的定向合成与克隆及其在哺乳动物细胞中的表达。
Virus Res. 1985 Dec;4(1):69-82. doi: 10.1016/0168-1702(85)90021-8.
5
Expression of unspliced NS1 mRNA, spliced NS2 mRNA, and a spliced chimera mRNA from cloned influenza virus NS DNA in an SV40 vector.在SV40载体中，来自克隆流感病毒NS DNA的未剪接NS1 mRNA、剪接NS2 mRNA和剪接嵌合mRNA的表达。
Virology. 1984 May;135(1):139-47. doi: 10.1016/0042-6822(84)90124-7.
6
Application of bioinformatics-coupled experimental analysis reveals a new transport-competent nuclear localization signal in the nucleoprotein of influenza A virus strain.生物信息学与实验分析相结合的应用揭示了甲型流感病毒株核蛋白中一种新的具有转运能力的核定位信号。
BMC Cell Biol. 2008 Apr 28;9:22. doi: 10.1186/1471-2121-9-22.
7
Location of influenza virus M, NP and NS1 proteins in microinjected cells.流感病毒M、NP和NS1蛋白在显微注射细胞中的定位。
J Gen Virol. 1985 Nov;66 ( Pt 11):2319-34. doi: 10.1099/0022-1317-66-11-2319.
8
Identification of the sequence responsible for the nuclear accumulation of the influenza virus nucleoprotein in Xenopus oocytes.在非洲爪蟾卵母细胞中负责流感病毒核蛋白核内积累的序列鉴定。
Cell. 1985 Mar;40(3):667-75. doi: 10.1016/0092-8674(85)90215-6.
9
Complete nucleotide sequence of the nucleoprotein gene of influenza B virus.乙型流感病毒核蛋白基因的完整核苷酸序列
J Virol. 1983 Sep;47(3):642-8. doi: 10.1128/JVI.47.3.642-648.1983.
10
Characterisation of an avian influenza virus nucleoprotein expressed in E. coli and in insect cells.
Arch Virol. 1990;113(3-4):267-77. doi: 10.1007/BF01316679.

引用本文的文献

1
Determining influenza virus shedding at different time points in madin-darby canine kidney cell line.确定甲型流感病毒在 Madin-Darby 犬肾细胞系中不同时间点的脱落情况。
Cell J. 2013 Summer;15(2):130-5. Epub 2013 Jul 2.
2
Temperature-sensitive lesions in two influenza A viruses defective for replicative transcription disrupt RNA binding by the nucleoprotein.两种在复制转录方面存在缺陷的甲型流感病毒中的温度敏感损伤会破坏核蛋白与RNA的结合。
J Virol. 1999 Sep;73(9):7349-56. doi: 10.1128/JVI.73.9.7349-7356.1999.
3
Nuclear trafficking of influenza virus ribonuleoproteins in heterokaryons.

本文引用的文献

1
The structure and composition of the myxoviruses. I. Electron microscope studies of the structure of myxovirus particles by negative staining techniques.黏液病毒的结构与组成。I. 运用负染色技术对黏液病毒颗粒结构进行的电子显微镜研究。
Virology. 1960 May;11:79-98. doi: 10.1016/0042-6822(60)90056-8.
2
Studies on influenza infection in ferrets by means of fluorescein-labelled antibody. II. The role of soluble antigen in nuclear fluorescence and cross-reactions.利用荧光素标记抗体对雪貂流感感染的研究。II. 可溶性抗原在核荧光和交叉反应中的作用。
J Exp Med. 1955 Jun 1;101(6):677-86. doi: 10.1084/jem.101.6.677.
3
The formation of fowl plague virus antigens in infected cells, as studied with fluorescent antibodies.
流感病毒核糖核蛋白在异核体中的核运输
J Virol. 1996 May;70(5):2743-56. doi: 10.1128/JVI.70.5.2743-2756.1996.
4
Complete nucleotide sequence of the nucleoprotein gene of influenza B virus.乙型流感病毒核蛋白基因的完整核苷酸序列
J Virol. 1983 Sep;47(3):642-8. doi: 10.1128/JVI.47.3.642-648.1983.
5
Combined action of mouse alpha and beta interferons in influenza virus-infected macrophages carrying the resistance gene Mx.小鼠α干扰素和β干扰素在携带抗性基因Mx的流感病毒感染巨噬细胞中的联合作用
J Virol. 1984 Mar;49(3):709-16. doi: 10.1128/JVI.49.3.709-716.1984.
6
Construction of cell lines that regulate by temperature the amplification and expression of influenza virus non-structural protein genes.构建能通过温度调节流感病毒非结构蛋白基因扩增和表达的细胞系。
EMBO J. 1986 Sep;5(9):2387-92. doi: 10.1002/j.1460-2075.1986.tb04508.x.
7
Expression of a functional influenza viral cap-recognizing protein by using a bovine papilloma virus vector.利用牛乳头瘤病毒载体表达功能性流感病毒帽识别蛋白。
Proc Natl Acad Sci U S A. 1985 Jul;82(13):4326-30. doi: 10.1073/pnas.82.13.4326.
8
Transport of incoming influenza virus nucleocapsids into the nucleus.传入的流感病毒核衣壳向细胞核的转运。
J Virol. 1991 Jan;65(1):232-44. doi: 10.1128/JVI.65.1.232-244.1991.
用荧光抗体研究感染细胞中禽瘟病毒抗原的形成。
Virology. 1957 Oct;4(2):328-45. doi: 10.1016/0042-6822(57)90067-3.
4
Studies of influenza virus infection in the chick embryo using fluorescent antibody.利用荧光抗体对鸡胚中的流感病毒感染进行的研究。
J Exp Med. 1954 May 1;99(5):419-28. doi: 10.1084/jem.99.5.419.
5
Localization of the Ts defect in two Ts mutants of influenza A virus: evidence for the occurrence of intracistronic complementation between Ts mutants of influenza A virus coding for the neuraminidase and nucleoprotein polypeptides.甲型流感病毒两个温度敏感（Ts）突变体中Ts缺陷的定位：编码神经氨酸酶和核蛋白多肽的甲型流感病毒Ts突变体之间发生顺反子内互补的证据。
Virology. 1980 Mar;101(2):484-92. doi: 10.1016/0042-6822(80)90462-6.
6
Influenza virus, an RNA virus, synthesizes its messenger RNA in the nucleus of infected cells.流感病毒是一种RNA病毒，在受感染细胞的细胞核中合成信使RNA。
Cell. 1981 Nov;26(3 Pt 1):391-400. doi: 10.1016/0092-8674(81)90208-7.
7
The structure of the gene encoding the nucleoprotein of human influenza virus A/PR/8/34.编码人甲型流感病毒A/PR/8/34核蛋白的基因结构。
Virology. 1981 Oct 30;114(2):423-8. doi: 10.1016/0042-6822(81)90223-3.
8
Migration of influenza virus-specific polypeptides from cytoplasm to nucleus of infected cells.流感病毒特异性多肽从受感染细胞的细胞质迁移至细胞核。
Virology. 1981 May;111(1):154-64. doi: 10.1016/0042-6822(81)90661-9.
9
The use of synthetic oligodeoxynucleotide primers in cloning and sequencing segment of 8 influenza virus (A/PR/8/34).合成寡脱氧核苷酸引物在克隆和测序8种流感病毒（A/PR/8/34）片段中的应用
Nucleic Acids Res. 1981 Jan 24;9(2):237-45. doi: 10.1093/nar/9.2.237.
10
Studies on the helical nucleocapsid of influenza virus.流感病毒螺旋核衣壳的研究。
Virology. 1982 Apr 30;118(2):466-70. doi: 10.1016/0042-6822(82)90367-1.