Egen-Schwind C, Eckard R, Kemper F H
Institut für Pharmakologie und Toxikologie, Westfälischen Wilhelms-Universität Münster, Federal Republic of Germany.
Planta Med. 1992 Aug;58(4):301-5. doi: 10.1055/s-2006-961471.
The metabolic and kinetic behaviour of different garlic (Allium sativum L., Alliaceae) constituents were investigated in the isolated perfused rat liver, using aqueous extracts of garlic powder as well as isolated allicin, the main product of the enzymatic degradation of alliin. Allicin (allyl thiosulfinate) showed a remarkable first pass effect and passed the liver unmetabolized only at high concentrations which caused considerable cell injuries. Diallyl disulfide and allyl mercaptan were identified as metabolites of allicin, whereby diallyl disulfide probably is the metabolic precursor of allyl mercaptan as shown by perfusion with diallyl disulfide alone. The metabolites diallyl disulfide and allyl mercaptan could be determined in the perfusion medium as well as in the bile and the liver tissue. Other degradation products of garlic were also investigated in this model. Ajoenes and vinyldithiins were detected in perfusion medium after liver passage but no metabolites of them could be identified up to now.
利用大蒜粉水提取物以及蒜氨酸酶促降解的主要产物——蒜素，在离体灌注大鼠肝脏中研究了不同大蒜（葱属植物大蒜，葱科）成分的代谢和动力学行为。蒜素（烯丙基硫代亚磺酸盐）表现出显著的首过效应，仅在高浓度时才会未经代谢地通过肝脏，而高浓度会导致相当程度的细胞损伤。二烯丙基二硫化物和烯丙基硫醇被鉴定为蒜素的代谢产物，仅用二烯丙基二硫化物灌注显示，二烯丙基二硫化物可能是烯丙基硫醇的代谢前体。代谢产物二烯丙基二硫化物和烯丙基硫醇可在灌注介质、胆汁和肝脏组织中检测到。在该模型中还研究了大蒜的其他降解产物。肝脏灌注后，在灌注介质中检测到了ajoenes和乙烯基二硫醚，但目前尚未鉴定出它们的代谢产物。
