Drowart A, Huygen K, Launois P, Jann E, Nyabenda J, van Vooren J P
Hôpital Erasme, ULB, Brussels, Belgium.
Scand J Immunol. 1994 Dec;40(6):643-7. doi: 10.1111/j.1365-3083.1994.tb03517.x.
Antigen 85 complex is the major protein component present in M. bovis BCG culture filtrate (CF). It consists of a family of three proteins: 85A, 85B and 85C. Combining isoelectric focusing and Western blot analysis, we have previously identified different antigenically related proteins present in the CF of other mycobacteria (M. tuberculosis, M. kansasii, M. avium, M. gordonae, M. fortuitum and M. phlei) using monoclonal antibodies (MoAbs) directed against the antigen 85 complex of M. bovis BCG. Humoral immune response directed against these cross-reactive homologues was analysed in sera from 20 patients with multibacillary leprosy (BL/LL), from 20 patients with paucibacillary leprosy (BT/TT) and from 15 healthy leprosy contacts. All the antigen 85 homologues identified in the seven CFs by MoAbs were also recognized by IgG present in sera from multibacillary leprosy patients, but not or very faintly in sera from paucibacillary leprosy patients or from healthy subjects. These results suggest that some of the M. leprae epitopes inducing a significant humoral response in multibacillary leprosy are common to the various 85 antigenically related proteins present in all mycobacterial species.
抗原85复合物是卡介苗培养滤液（CF）中的主要蛋白质成分。它由三种蛋白质组成的一个家族：85A、85B和85C。结合等电聚焦和蛋白质印迹分析，我们先前使用针对卡介苗抗原85复合物的单克隆抗体（MoAbs），鉴定了其他分枝杆菌（结核分枝杆菌、堪萨斯分枝杆菌、鸟分枝杆菌、戈登分枝杆菌、偶然分枝杆菌和草分枝杆菌）CF中存在的不同抗原相关蛋白。在20例多菌型麻风病（BL/LL）患者、20例少菌型麻风病（BT/TT）患者和15名健康麻风病接触者的血清中，分析了针对这些交叉反应同源物的体液免疫反应。通过MoAbs在七种CF中鉴定出的所有抗原85同源物，也被多菌型麻风病患者血清中的IgG识别，但在少菌型麻风病患者或健康受试者的血清中未被识别或识别非常微弱。这些结果表明，在多菌型麻风病中诱导显著体液反应的一些麻风杆菌表位，对于所有分枝杆菌物种中存在的各种85抗原相关蛋白是共同的。
