Bartolucci S, Lancieri M, di Jeso F, de Vincentiis M
C R Seances Soc Biol Fil. 1977;171(3):512-6.
Improved methods of separation, identification and determination of phosphagens have been applied to the study of monosubstituted guanidines and phosphagenes in sea urchin eggs, before and after fertilization. We have been able not only to identify in these materials phosphoarginine, but also phosphocreatine and to detect an unknown phosphagen. After fertilization, free arginine and creatine decrease, within respect to unfertilized eggs, whereas phosphocreatine undergoes a large increase; phosphoarginine remains almost constant, and an unknown phosphagen appears. These observations allow to interprete the results of some authors, who, applyng less refined methods, found only an increase in phosphoarginine in the same materials.
改进的磷酸肌酸分离、鉴定和测定方法已应用于研究海胆卵受精前后的单取代胍和磷酸肌酸。我们不仅能够在这些材料中鉴定出磷酸精氨酸，还能鉴定出磷酸肌酸，并检测到一种未知的磷酸肌酸。受精后，与未受精卵相比，游离精氨酸和肌酸减少，而磷酸肌酸大幅增加；磷酸精氨酸几乎保持不变，并且出现了一种未知的磷酸肌酸。这些观察结果有助于解释一些作者的研究结果，他们使用的方法不够精细，在相同材料中只发现了磷酸精氨酸的增加。
