Gao X, Bhatia K, Trent R J, Serjeantson S W
Human Genetics Group, John Curtin School of Medical Research, Australian National University.
Tissue Antigens. 1992 Jul;40(1):31-7. doi: 10.1111/j.1399-0039.1992.tb01954.x.
HLA-DRB1 nucleotide sequence polymorphisms have been examined in 304 Melanesians from the Papua New Guinean coast (Madang), islands (Rabaul) and highlands (Goroka), and from New Caledonia and Fiji. A total of 20 HLA-DRB1 alleles were detected by oligonucleotide hybridizations of exon 2 HLA-DRB1 polymerase chain reaction products, in a typing protocol designed to detect all 42 officially-designated HLA-DRB1 alleles. DRB11502 and 1101 alleles were the most common alleles in coastal and island Melanesians, while DRB11501, 1502 and 1408 predominated in Papua New Guinean highlanders. Undefined mixed lymphocyte reaction determinants in earlier studies of Melanesians could be accounted for in the present study as DRB10410, 1407 and 1408 in Papua New Guinean highlanders and as DRB11104 and 1602 in coastal people. Nucleotide sequence polymorphisms at HLA-DQA1, -DQB1, -DRB3 and -DRB5 were also determined for estimating HLA-DR,DQ allelic disequilibrium relationships; unusual haplotypes in Melanesians included DBR11502, DRB50101 and DRB10410, DQB10402. Previous claims of limited heterogeneity in the HLA-DR allele repertoire in Melanesians are now seen to reflect limitations of early typing reagents rather than any dramatic restriction in HLA-DR allelic diversity.
对来自巴布亚新几内亚海岸（马当）、岛屿（拉包尔）和高地（戈罗卡）以及新喀里多尼亚和斐济的304名美拉尼西亚人进行了HLA - DRB1核苷酸序列多态性检测。通过对HLA - DRB1聚合酶链反应产物外显子2进行寡核苷酸杂交，在一个旨在检测所有42个官方指定的HLA - DRB1等位基因的分型方案中，共检测到20个HLA - DRB1等位基因。DRB11502和1101等位基因是沿海和岛屿美拉尼西亚人中最常见的等位基因，而DRB11501、1502和1408在巴布亚新几内亚高地人中占主导地位。在美拉尼西亚人早期研究中未明确的混合淋巴细胞反应决定因素，在本研究中可解释为巴布亚新几内亚高地人中的DRB10410、1407和1408，以及沿海人群中的DRB11104和1602。还确定了HLA - DQA1、- DQB1、- DRB3和- DRB5的核苷酸序列多态性，以估计HLA - DR、DQ等位基因不平衡关系；美拉尼西亚人中不寻常的单倍型包括DBR11502、DRB50101和DRB10410、DQB10402。现在看来，之前关于美拉尼西亚人HLA - DR等位基因库中有限异质性的说法反映了早期分型试剂的局限性，而非HLA - DR等位基因多样性存在任何显著限制。
