Enwemeka C S
Department of Orthopaedics and Rehabilitation, University of Miami School of Medicine and Research Service, Veterans Affairs Medical Center, FL 33146.
Tissue Cell. 1992;24(4):511-23. doi: 10.1016/0040-8166(92)90067-h.
Collagen fibrils are not found in fibroblast cytoplasm except in certain pathological conditions or in the presence of drugs and other agents that accelerate collagen turnover. Because low energy laser photostimulation is both a non-pathogenic and non-chemical accelerator of collagen synthesis, its effects were studied on four groups of calcaneal tendons from 18 rabbits (1) to test the hypothesis that vacuolar fibrils are not produced exclusively by diseases and chemical agents, and (2) to compare the morphometry of matrical and vacuolar fibrils. The right calcaneal tendons of nine rabbits were surgically tenotomized and repaired; six of these were transcutaneously irradiated with He:Ne laser everyday. The right calcaneal tendon of six of the remaining nine rabbits were similarly irradiated with laser, but without prior tenotomy and repair. 21 days later, all tendons were fixed in situ and processed for electron microscopy. Fibril-bearing vacuoles were found only in fibroblasts of tenotomized laser-irradiated tendons. Similar vacuoles were not seen in non-tenotomized laser-irradiated tendons nor in non-irradiated tendons whether tenotomized or not. Mann-Whitney U tests revealed no statistically significant differences in the cross-sectional areas or diameters of matrical and vacuolar fibrils. These findings suggest (a) that matrical and vacuolar fibrils have a common origin, and (b) that vacuolar fibrils can be induced by a non-pathologic, non-chemical accelerator of collagen synthesis.
除了在某些病理状况下，或存在加速胶原蛋白周转的药物及其他因子时，在成纤维细胞胞质中找不到胶原纤维。由于低能量激光光刺激是胶原蛋白合成的一种非致病性且非化学性的促进剂，因此对18只兔子的四组跟腱进行了其效果的研究，目的如下：（1）检验空泡状纤维并非仅由疾病和化学因子产生这一假说；（2）比较基质纤维和空泡状纤维的形态计量学。对9只兔子的右跟腱进行手术切断并修复；其中6只每天经皮用氦氖激光照射。其余9只兔子中，6只兔子的右跟腱同样接受激光照射，但未事先进行切断和修复。21天后，所有跟腱原位固定并进行电子显微镜处理。仅在切断后接受激光照射的跟腱的成纤维细胞中发现了含纤维的空泡。在未切断而接受激光照射的跟腱中，以及无论是否切断的未照射跟腱中均未见到类似空泡。曼-惠特尼U检验显示，基质纤维和空泡状纤维的横截面积或直径无统计学显著差异。这些发现表明：（a）基质纤维和空泡状纤维有共同起源；（b）空泡状纤维可由胶原蛋白合成的非病理性、非化学性促进剂诱导产生。
