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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Wanderer C, Buchi D F, Tassini C M, Raiser A G, Schimitt I

Departamento de Biologia Celular, Universidade Federal do Paraná, Curitiba, PR, Brasil.

Braz J Med Biol Res. 1994 Sep;27(9):2241-51.

Lectins labeled with colloidal gold particles were used for the ultrastructural evaluation of the biological effects of GaAs softlaser irradiation on the healing of dog tendon wounds. 2. Six dogs were submitted to tenotomy and tenorrhaphy on the right and left hind legs. All animals received laser irradiation (4 J/cm2) daily for ten days only on the left leg, and the right leg of the same animal was used as control. Biopsies were taken 11, 22 and 40 days after surgery. 3. Laser-irradiated and control tendon tissues were embedded in L.R. White resin and thin sections were incubated in the presence of gold-labeled Arachis hypogaea (PNA), Canavalia ensiformes (Con A) and Triticum vulgare (WGA). 4. In general, the control and laser-irradiated tissues presented homogeneous and similar labelling of heterochromatin, rough endoplasmic reticulum and extracellular matrix. 5. We conclude that GaAs softlaser irradiation does not produce significant changes in the glycosylation of healing tendons.

用胶体金颗粒标记的凝集素对砷化镓软激光照射犬肌腱伤口愈合的生物学效应进行超微结构评估。2. 对6只犬的左右后腿进行肌腱切断术和肌腱缝合术。所有动物仅在左腿上每天接受10天的激光照射（4 J/cm²），同一动物的右腿用作对照。术后11、22和40天取活检组织。3. 将激光照射组和对照组的肌腱组织包埋在L.R. White树脂中，薄切片在金标记的花生凝集素（PNA）、刀豆凝集素（Con A）和小麦胚凝集素（WGA）存在下孵育。4. 一般来说，对照组和激光照射组的组织在异染色质、粗面内质网和细胞外基质的标记方面呈现出均匀且相似的情况。5. 我们得出结论，砷化镓软激光照射不会使愈合中的肌腱糖基化产生显著变化。

Wanderer C, Buchi D F, Tassini C M, Raiser A G, Schimitt I

Departamento de Biologia Celular, Universidade Federal do Paraná, Curitiba, PR, Brasil.

Braz J Med Biol Res. 1994 Sep;27(9):2241-51.

Lectins labeled with colloidal gold particles were used for the ultrastructural evaluation of the biological effects of GaAs softlaser irradiation on the healing of dog tendon wounds. 2. Six dogs were submitted to tenotomy and tenorrhaphy on the right and left hind legs. All animals received laser irradiation (4 J/cm2) daily for ten days only on the left leg, and the right leg of the same animal was used as control. Biopsies were taken 11, 22 and 40 days after surgery. 3. Laser-irradiated and control tendon tissues were embedded in L.R. White resin and thin sections were incubated in the presence of gold-labeled Arachis hypogaea (PNA), Canavalia ensiformes (Con A) and Triticum vulgare (WGA). 4. In general, the control and laser-irradiated tissues presented homogeneous and similar labelling of heterochromatin, rough endoplasmic reticulum and extracellular matrix. 5. We conclude that GaAs softlaser irradiation does not produce significant changes in the glycosylation of healing tendons.

用胶体金颗粒标记的凝集素对砷化镓软激光照射犬肌腱伤口愈合的生物学效应进行超微结构评估。2. 对6只犬的左右后腿进行肌腱切断术和肌腱缝合术。所有动物仅在左腿上每天接受10天的激光照射（4 J/cm²），同一动物的右腿用作对照。术后11、22和40天取活检组织。3. 将激光照射组和对照组的肌腱组织包埋在L.R. White树脂中，薄切片在金标记的花生凝集素（PNA）、刀豆凝集素（Con A）和小麦胚凝集素（WGA）存在下孵育。4. 一般来说，对照组和激光照射组的组织在异染色质、粗面内质网和细胞外基质的标记方面呈现出均匀且相似的情况。5. 我们得出结论，砷化镓软激光照射不会使愈合中的肌腱糖基化产生显著变化。