MOULE Y, ROUILLER C, CHAUVEAU J
J Biophys Biochem Cytol. 1960 Jun;7(3):547-58. doi: 10.1083/jcb.7.3.547.
Microsomes isolated by differential centrifugation from a rat liver homogenate in 0.88 M sucrose solution have been studied from the biochemical and morphological point of view. 1. Under these experimental conditions, the "total microsome" fraction was obtained by centrifuging the cytoplasmic extract free of nuclei and mitochondria, for 3 hours at 145,000 g. Morphologically, the total microsomes consist mainly of "rough-surfaced membranes" and "smooth" ones. 2. The total microsomes have been divided into 2 subfractions so that the 1st microsomal fraction contains the "rough" vesicles (2 hours centrifugation at 40,000 g) while the 2nd microsomal fraction consists essentially of smooth vesicles, free particles, and ferritin (centrifugation of the supernatant at 145,000 g for 3 hours). 3. By the action of 0.4 per cent sodium deoxycholate in 0.88 M sucrose, it was possible to obtain a pellet for each of the 2 fractions which consisted of dense particles, rich in RNA, poor in lipids, and which represented about 50 to 60 percent of the RNA and 10 to 15 per cent of the proteins. The results have been discussed taking into consideration the hypothesis of the presence of RNA in the membranes of microsomal vesicles.
已从生化和形态学角度研究了通过差速离心法从处于0.88 M蔗糖溶液中的大鼠肝脏匀浆中分离出的微粒体。1. 在这些实验条件下，通过将不含细胞核和线粒体的细胞质提取物在145,000 g下离心3小时获得“总微粒体”部分。从形态学上看，总微粒体主要由“糙面内质网”和“光面内质网”组成。2. 总微粒体已被分为2个亚组分，使得第一微粒体组分包含“糙面”小泡（在40,000 g下离心2小时），而第二微粒体组分主要由光面小泡、游离颗粒和铁蛋白组成（将上清液在145,000 g下离心3小时）。3. 通过在0.88 M蔗糖中加入0.4%的脱氧胆酸钠，有可能从这2个组分中分别获得一个沉淀，该沉淀由富含RNA、脂质含量低的致密颗粒组成，其RNA含量约占50%至60%，蛋白质含量约占10%至15%。已结合微粒体小泡膜中存在RNA的假说对结果进行了讨论。
