大鼠肝脏微粒体和分离的亚细胞膜的分析研究。VI. 利用铁蛋白标记抗体对微粒体进行细胞色素b5的电子显微镜检查。
Analytical study of microsomes and isolated subcellular membranes from rat liver. VI. Electron microscope examination of microsomes for cytochrome b5 by means of a ferritin-labeled antibody.
作者信息
Remacle J, Fowler S, Beaufay H, Amarcostesec A, Berthet J
出版信息
J Cell Biol. 1976 Nov;71(2):551-64. doi: 10.1083/jcb.71.2.551.
Abstract
The distribution of cytochrome b5 in rat liver microsomes, and in two microsomal subfractions isolated by density equilibration in a linear sucrose gradient, was studied under the electron microscope by means of a ferritin-labeled hybrid anti-cytochrome b5/anti-ferritin antibody. Results of this study show that cytochrome b5 is present in essentially all microsomal vesicles derived from endoplasmic reticulum (ER), whether rough or smooth. Thus, the dissociation of ER constituents into two groups (b and c), achieved by subfractionating microsomes by isopycnic centrifugation (Beaufay, H., A. Amar-Costesec, D. Thines-Sempoux, M. Wibo, M. Robbi, and J. Berthet. 1974. J. Cell Biol. 61:213-231), does not reflect the association of each group with distinct microsomal particles but reflects rather an enzymatic heterogeneity of the ER: the ratio of group c to group b enzymes increasing with the density and ribosome load of the particles.
摘要
利用铁蛋白标记的抗细胞色素b5/抗铁蛋白杂交抗体，在电子显微镜下研究了细胞色素b5在大鼠肝脏微粒体以及通过线性蔗糖梯度密度平衡分离得到的两个微粒体亚组分中的分布。这项研究结果表明，细胞色素b5基本上存在于源自内质网（ER）的所有微粒体小泡中，无论其是粗面还是滑面的。因此，通过等密度离心对微粒体进行亚分级分离（博法伊，H.，A. 阿马尔 - 科斯泰塞克，D. 蒂内斯 - 桑普克斯，M. 维博，M. 罗比，以及J. 贝尔泰。1974年。《细胞生物学杂志》61:213 - 231），将ER成分分离成两组（b组和c组），这并非反映每组与不同微粒体颗粒的关联，而是反映了ER的酶学异质性：c组酶与b组酶的比例随着颗粒的密度和核糖体负载量增加而增加。
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