Yeh J, Rosen K M, Villa-Komaroff L
Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women's Hospital, Boston, MA 02115.
Am J Obstet Gynecol. 1992 Jul;167(1):242-5. doi: 10.1016/s0002-9378(11)91666-4.
Little is known concerning the presence of transforming growth factor-alpha in fetal and neonatal tissues. Our objective was to analyze messenger ribonucleic acid expression for transforming growth factor-alpha and epidermal growth factor, the structural and biochemical analog of transforming growth factor-alpha in fetal and neonatal murine brain.
Messenger ribonucleic acid was prepared from whole brains of mice from these developmental stages: embryonic days 14, 15, and 17, postnatal days 0, 4, 10, and adult. Polymerase chain reaction was performed on the complementary deoxyribonucleic acid obtained from reverse transcription of messenger ribonucleic acid with transforming growth factor-alpha and epidermal growth factor primers. In addition, ribonuclease protection assay was used to identify transforming growth factor-alpha transcripts.
We found messenger ribonucleic acid encoding transforming growth factor-alpha in all stages of development used. Epidermal growth factor messenger ribonucleic acid was not found in any stage. Ribonuclease protection assay confirmed transforming growth factor-alpha transcripts in these tissues.
Transforming growth factor-alpha may play an autocrine or paracrine role in the differentiation or maintenance of murine fetal and neonatal brains.
关于胎儿和新生儿组织中转化生长因子-α的存在情况，人们了解甚少。我们的目的是分析胎儿和新生小鼠大脑中转化生长因子-α及其结构和生化类似物表皮生长因子的信使核糖核酸表达。
从处于这些发育阶段的小鼠全脑中提取信使核糖核酸：胚胎第14、15和17天，出生后第0、4、10天以及成年期。使用转化生长因子-α和表皮生长因子引物，对从信使核糖核酸逆转录得到的互补脱氧核糖核酸进行聚合酶链反应。此外，采用核糖核酸酶保护试验来鉴定转化生长因子-α转录本。
我们在所使用的所有发育阶段均发现了编码转化生长因子-α的信使核糖核酸。在任何阶段均未发现表皮生长因子信使核糖核酸。核糖核酸酶保护试验证实了这些组织中存在转化生长因子-α转录本。
转化生长因子-α可能在小鼠胎儿和新生儿大脑的分化或维持中发挥自分泌或旁分泌作用。
