Gharib-Hamrouche N, Chêne N, Martal J
INRA, station de physiologie animale, unité d'endocrinologie de l'embryon, Jouy-en-Josas, France.
Reprod Nutr Dev. 1995;35(3):291-303. doi: 10.1051/rnd:19950305.
Control of growth and differentiation during mammalian embryogenesis may be regulated by growth factors. With the use of a very sensitive method, the reverse transcription-polymerase chain reaction, expression of some growth factor transcripts was examined during ovine peri-implantation development. Transcripts for transforming growth factor-alpha (TGF-alpha) were found in day 15 to day 30 conceptuses, and in uterine tissues. Epidermal growth factor (EGF) was not detectable in any ovine stages or tissues studied. TGF-alpha could be the normal physiological ligand for the epidermal growth factor receptor present on the trophoblastic tissues, and its expression pattern suggests an autocrine and paracrine role in the growth and differentiation of ovine embryos.
哺乳动物胚胎发生过程中的生长和分化控制可能受生长因子调节。运用一种非常灵敏的方法——逆转录-聚合酶链反应，检测了绵羊着床前后发育过程中一些生长因子转录本的表达情况。在妊娠第15天至第30天的胚胎以及子宫组织中发现了转化生长因子-α（TGF-α）的转录本。在所研究的任何绵羊阶段或组织中均未检测到表皮生长因子（EGF）。TGF-α可能是滋养层组织上存在的表皮生长因子受体的正常生理配体，其表达模式表明它在绵羊胚胎的生长和分化中具有自分泌和旁分泌作用。
