Neri A, Paciucci P A, Nobile F, Brugiatelli M, Camba L, Comis M, Iacopino P, Trapani Lombardo V
Ric Clin Lab. 1977 Apr-Jun;7(2):124-35. doi: 10.1007/BF02879478.
The peripheral blood lymphocytes of 13 previously untreated chronic lymphocytic leukemia (CLL) patients showed a decreased and delayed response in vitro to plant mitogens (PHA and PWM) and specific antigens (PPD and MLC). In addition, the serum of these patients inhibited the mitotic reactivity of both autologous and homologous normal lymphocytes. Since incubation with CLL serum did not affect the SRBC-rosetting capacity of normal lymphocytes, we believe that CLL serum interferes with some metabolic stage in blastogenesis rather than at the level of mitogen membrane interaction. The in vitro transformation of lymph node, bone marrow and peripheral blood lymphocytes in some of these patients was also investigated. Stimulation of peripheral blood cells with plant mitogens resulted in a more serious impairment of the response than that found with bone marrow and lymph node cells. This could be explained by the fact that, although CLL is a widespread lymphoproliferative disorder, some preferential homing of normally reactive cells in the bone marrow and lymph nodes cannot be excluded. Finally, since no stimulation was observed in mixed leukocyte cultures (MLC), our experiments provide evidence that no antigenic differences are detectable in CLL lymphoid populations.
13例未经治疗的慢性淋巴细胞白血病（CLL）患者的外周血淋巴细胞在体外对植物有丝分裂原（PHA和PWM）和特异性抗原（PPD和MLC）的反应降低且延迟。此外，这些患者的血清抑制自体和同源正常淋巴细胞的有丝分裂反应性。由于与CLL血清孵育不影响正常淋巴细胞的SRBC玫瑰花结形成能力，我们认为CLL血清干扰了母细胞生成的某些代谢阶段，而非有丝分裂原膜相互作用水平。还对其中一些患者的淋巴结、骨髓和外周血淋巴细胞的体外转化进行了研究。用植物有丝分裂原刺激外周血细胞导致的反应损伤比骨髓和淋巴结细胞更严重。这可以用以下事实来解释，即尽管CLL是一种广泛的淋巴细胞增殖性疾病，但不能排除正常反应性细胞在骨髓和淋巴结中有一些优先归巢的情况。最后，由于在混合淋巴细胞培养（MLC）中未观察到刺激，我们的实验提供了证据，表明在CLL淋巴细胞群体中未检测到抗原差异。
