Isaković K, Lenert G
Blood Cells. 1987;12(2):355-64.
The ability of leukemic B lymphocytes to proliferate after in vitro stimulation with PWM and PHA was studied in 15 patients with chronic lymphocytic leukemia. Peripheral blood lymphocytes of five healthy subjects as well as purified normal B lymphocytes were used as controls. Leukemic lymphocytes of all donors expressed the same membrane phenotype, M receptor, and B7 and Ia antigens. The lymphocyte populations investigated were not completely free from myelomonocytic cells and contained small numbers of T lymphocytes. DNA synthesis was determined on days 3, 5, and 7 of culture by measuring the incorporation of tritiated thymidine. PWM-induced proliferation of leukemic B lymphocytes of nine patients was within normal limits, while the response of leukemic cells of six patients was very low. On the other hand, all CLL donors responded very well to PHA. Moreover, the response of leukemic B lymphocytes was significantly higher than the response of normal B cells. It was concluded that leukemic B lymphocytes of CLL patients are capable of proliferation after stimulation with PWM and PHA. The mechanisms underlying these responses to PWM and PHA are likely to be different.
对15例慢性淋巴细胞白血病患者的白血病B淋巴细胞在体外经PWM和PHA刺激后增殖的能力进行了研究。以5名健康受试者的外周血淋巴细胞以及纯化的正常B淋巴细胞作为对照。所有供者的白血病淋巴细胞均表达相同的膜表型、M受体以及B7和Ia抗原。所研究的淋巴细胞群体并非完全不含髓单核细胞，且含有少量T淋巴细胞。通过测量氚标记胸腺嘧啶核苷的掺入量，在培养的第3天、第5天和第7天测定DNA合成。9例患者的白血病B淋巴细胞经PWM诱导的增殖在正常范围内，而6例患者的白血病细胞反应非常低。另一方面，所有慢性淋巴细胞白血病供者对PHA反应良好。此外，白血病B淋巴细胞的反应明显高于正常B细胞。得出的结论是，慢性淋巴细胞白血病患者的白血病B淋巴细胞在经PWM和PHA刺激后能够增殖。这些对PWM和PHA反应的潜在机制可能不同。
