Herrmann F, Kolecki P, Sieber G, Ludwig W D, Komischke B, Rühl H
Am J Clin Pathol. 1985 Feb;83(2):249-53. doi: 10.1093/ajcp/83.2.249.
A case of chronic lymphocytic leukemia is described in which large numbers of peripheral blood lymphocytes expressed immunoglobulin on their membrane and rosetted spontaneously with sheep red blood cells (SRBC) at 4 degrees C. They also showed weak staining with a heterologous antiserum against T-cells as well as with a monoclonal antibody (OKT11) with specificity for an epitope associated with the SRBC-receptor, but failed to react with other T-lineage-restricted monoclonal antibodies. The B-cell origin of the leukemic cells was documented by the presence of light-chain-restricted monoclonal surface immunoglobulin, reactivity with various monoclonal anti-B-cell reagents, presence of Ia-like antigens, their capability to synthesize intracytoplasmic immunoglobulin on exposure to phorbol ester TPA, their lack of response to T-cell mitogen PHA, and their inability to help or suppress the allogeneic B-cell response upon PWM stimulation. Extensive blocking studies with both specific antisera and Forsmann antigen-rich guinea pig kidney extracts, which did not prevent SRBC-rosetting, lend support to the hypothesis that SRBC-rosette formation in this case was not attributable to an anti-SRBC affinity of the surface immunoglobulin on the cells. This case will be discussed in relation to the recent finding of SRBC-rosette expression on some cultured chronic lymphocytic leukemia B-lymphocytes.
本文描述了一例慢性淋巴细胞白血病病例，其中大量外周血淋巴细胞在其细胞膜上表达免疫球蛋白，并在4℃时与绵羊红细胞（SRBC）自发形成玫瑰花结。它们还用针对T细胞的异种抗血清以及对与SRBC受体相关的表位具有特异性的单克隆抗体（OKT11）进行了弱染色，但未能与其他T系限制性单克隆抗体发生反应。白血病细胞的B细胞起源通过轻链限制性单克隆表面免疫球蛋白的存在、与各种单克隆抗B细胞试剂的反应性、Ia样抗原的存在、它们在暴露于佛波酯TPA时合成胞浆内免疫球蛋白的能力、它们对T细胞有丝分裂原PHA的无反应以及它们在PWM刺激下无法辅助或抑制同种异体B细胞反应来证明。用特异性抗血清和富含福斯曼抗原的豚鼠肾提取物进行的广泛阻断研究，并未阻止SRBC玫瑰花结的形成，这支持了该病例中SRBC玫瑰花结形成并非归因于细胞表面免疫球蛋白对SRBC的亲和力这一假说。将结合最近在一些培养的慢性淋巴细胞白血病B淋巴细胞上发现的SRBC玫瑰花结表达来讨论该病例。
