Sastre J, Asensi M, Gascó E, Pallardó F V, Ferrero J A, Furukawa T, Viña J
Departamento de Fisiologia, Universidad de Valencia, Spain.
Am J Physiol. 1992 Nov;263(5 Pt 2):R992-5. doi: 10.1152/ajpregu.1992.263.5.R992.
We have studied the effect of exhaustive concentric physical exercise on glutathione redox status and the possible relationship between blood glutathione oxidation and blood lactate and pyruvate levels. Levels of oxidized glutathione (GSSG) in blood increase after exhaustive concentric physical exercise in trained humans. GSSG levels were 72% higher immediately after exercise than at rest. They returned to normal values 1 h after exercise. Blood reduced glutathione (GSH) levels did not change significantly after the exercise. We have found a linear relationship between GSSG-to-GSH and lactate-to-pyruvate ratios in human blood before, during, and after exhaustive exercise. In rats, physical exercise also caused an increase in blood GSSG levels that were 200% higher after physical exercise than at rest. GSH levels did not change significantly. Thus, both in rats and humans, exhaustive physical exercise causes a change in glutathione redox status in blood. We have also found that antioxidant administration, i.e., oral vitamin C, N-acetyl-L-cysteine, or glutathione, is effective in preventing oxidation of the blood glutathione pool after physical exercise in rats.
我们研究了力竭性向心体育锻炼对谷胱甘肽氧化还原状态的影响，以及血液中谷胱甘肽氧化与血液乳酸和丙酮酸水平之间的可能关系。在经过训练的人体中，力竭性向心体育锻炼后血液中氧化型谷胱甘肽（GSSG）水平会升高。运动后即刻GSSG水平比静息时高72%。运动1小时后它们恢复到正常水平。运动后血液中还原型谷胱甘肽（GSH）水平没有显著变化。我们发现在力竭性运动前、运动期间和运动后，人体血液中GSSG与GSH的比值和乳酸与丙酮酸的比值之间存在线性关系。在大鼠中，体育锻炼也会导致血液中GSSG水平升高，运动后比静息时高200%。GSH水平没有显著变化。因此，无论是在大鼠还是人体中，力竭性体育锻炼都会导致血液中谷胱甘肽氧化还原状态发生变化。我们还发现，给予抗氧化剂，即口服维生素C、N-乙酰-L-半胱氨酸或谷胱甘肽，对预防大鼠体育锻炼后血液谷胱甘肽池的氧化是有效的。
