Gohil K, Viguie C, Stanley W C, Brooks G A, Packer L
Department of Physiology-Anatomy, University of California, Berkeley 94720.
J Appl Physiol (1985). 1988 Jan;64(1):115-9. doi: 10.1152/jappl.1988.64.1.115.
To examine the effects of increased O2 utilization on the glutathione antioxidant system in blood, eight moderately trained male volunteers were exercised to peak O2 consumption (VO2peak) and for 90 min at 65% of VO2peak on a cycle ergometer. Blood samples were taken during exercise, and for up to 4 days of recovery from submaximal exercise. During exercise to VO2peak, blood reduced glutathione (GSH) and total glutathione [GSH + oxidized glutathione (GSSG)] did not change significantly. Lactate (L), pyruvate (P), and L/P increased significantly from rest values (P less than 0.01). During prolonged submaximal exercise, GSH decreased 60% from control, and GSSG increased 100%. Total glutathione, glucose, pyruvate, and lactate concentrations and L/P did not change significantly during sustained exercise. During recovery, GSH and GSH/GSSG increased from exercise levels and significantly overshot preexercise levels, reaching maximum values after 3 days. Oxidation of GSH during submaximal exercise and its reduction in recovery suggest increased formation of active O2-. species in blood during physical exercise in moderately trained males.
为研究增加氧气利用对血液中谷胱甘肽抗氧化系统的影响，八名中度训练的男性志愿者在自行车测力计上进行运动，直至达到最大耗氧量（VO2peak），并在65%VO2peak强度下持续运动90分钟。在运动期间以及从次最大运动恢复后的4天内采集血样。在达到VO2peak的运动过程中，血液中还原型谷胱甘肽（GSH）和总谷胱甘肽[GSH + 氧化型谷胱甘肽（GSSG）]无显著变化。乳酸（L）、丙酮酸（P）以及L/P较静息值显著升高（P<0.01）。在长时间次最大运动期间，GSH较对照组降低60%，GSSG升高100%。在持续运动期间，总谷胱甘肽、葡萄糖、丙酮酸、乳酸浓度以及L/P无显著变化。在恢复期间，GSH以及GSH/GSSG从运动水平升高，并显著超过运动前水平，在3天后达到最大值。次最大运动期间GSH的氧化及其在恢复过程中的还原表明，中度训练男性在体育锻炼期间血液中活性氧物种的生成增加。
