Agarwal A, Ozturk E, Loughlin K R
Division of Urology and Reproductive-Endocrinology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115.
Andrologia. 1992 Nov-Dec;24(6):327-9. doi: 10.1111/j.1439-0272.1992.tb02663.x.
This study was designed to assess the reproducibility of the computer-assisted semen analysis (CASA) system. Semen specimens from 41 suspected subfertile patients were analysed on two identical Hamilton-Thorn Motility analysers (HTM, model 2030, version 7). Specimens were analysed after liquefaction at 37 degrees C for 30 min on two HTMs adjusted to the same gate settings and by using the same semen droplet loaded on a Makler counting chamber prewarmed at 37 degrees C. Significant differences were seen in the total sperm count (P < 0.0004), motile sperm count (P < 0.004), amplitude of lateral head displacement (P < 0.0001), linearity (P < 0.01), and beat cross frequency (P < 0.0001) between the two HTMs. No significant differences between the two machines was seen in the case of other sperm motion parameters: percentage motility, average path velocity, straightline velocity, curvilinear velocity, straightness, and progressive motility. Our results indicate that the reproducibility of several semen parameters analysed by HTM is poor, and steps to remedy this problem are necessary.
本研究旨在评估计算机辅助精液分析（CASA）系统的可重复性。对41例疑似亚生育力患者的精液标本在两台相同的汉密尔顿-桑恩运动分析仪（HTM，型号2030，版本7）上进行分析。标本在37℃液化30分钟后，在两台调整到相同门控设置的HTM上进行分析，并使用加载在预热至37℃的马克勒计数室中的相同精液滴。两台HTM之间在总精子数（P < 0.0004）、活动精子数（P < 0.004）、头部侧移幅度（P < 0.0001）、直线性（P < 0.01）和鞭打交叉频率（P < 0.0001）方面存在显著差异。在其他精子运动参数方面，两台机器之间未观察到显著差异：活动率、平均路径速度、直线速度、曲线速度、直线度和进行性活动。我们的结果表明，HTM分析的几个精液参数的可重复性较差，有必要采取措施纠正这一问题。
