Choquet C G, Patel G B, Beveridge T J, Sprott G D
Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario.
Appl Environ Microbiol. 1992 Sep;58(9):2894-900. doi: 10.1128/aem.58.9.2894-2900.1992.
Unilamellar liposomes were formed by controlled detergent dialysis of mixed micelles consisting of acetone-insoluble total polar lipids extracted from various methanogens and the detergent n-octyl-beta-D-glucopyranoside. The final liposome populations were studied by dynamic light scattering and electron microscopy. Unilamellar liposomes with mean diameters smaller than 100 nm were obtained with lipid extracts of Methanococcus voltae, Methanosarcina mazei, Methanosaeta concilii, and Methanococcus jannaschii (grown at 50 degrees C), whereas larger (greater than 100-nm) unilamellar liposomes were obtained with lipid extracts of M. jannaschii grown at 65 degrees C. These liposomes were shown to be closed intact vesicles capable of retaining entrapped [14C]sucrose for extended periods of time. With the exception of Methanospirillum hungatei liposomes, all size distributions of the different liposome populations were fairly homogeneous.
单层脂质体是通过对混合胶束进行可控去污剂透析形成的，混合胶束由从各种产甲烷菌中提取的丙酮不溶性总极性脂质和去污剂正辛基-β-D-吡喃葡萄糖苷组成。通过动态光散射和电子显微镜研究最终的脂质体群体。用沃氏甲烷球菌、马氏甲烷八叠球菌、温和甲烷鬃菌和詹氏甲烷球菌（在50℃下生长）的脂质提取物获得了平均直径小于100nm的单层脂质体，而用在65℃下生长的詹氏甲烷球菌的脂质提取物获得了更大（大于100nm）的单层脂质体。这些脂质体被证明是封闭的完整囊泡，能够长时间保留包裹的[14C]蔗糖。除了亨氏甲烷螺菌脂质体，不同脂质体群体的所有大小分布都相当均匀。
