Felgner P L, Gadek T R, Holm M, Roman R, Chan H W, Wenz M, Northrop J P, Ringold G M, Danielsen M
Institute of Bio-Organic Chemistry, Syntex Research, Palo Alto, CA 94303.
Proc Natl Acad Sci U S A. 1987 Nov;84(21):7413-7. doi: 10.1073/pnas.84.21.7413.
A DNA-transfection protocol has been developed that makes use of a synthetic cationic lipid, N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTMA). Small unilamellar liposomes containing DOTMA interact spontaneously with DNA to form lipid-DNA complexes with 100% entrapment of the DNA, DOTMA facilitates fusion of the complex with the plasma membrane of tissue culture cells, resulting in both uptake and expression of the DNA. The technique is simple, highly reproducible, and effective for both transient and stable expression of transfected DNA. Depending upon the cell line, lipofection is from 5- to greater than 100-fold more effective than either the calcium phosphate or the DEAE-dextran transfection technique.
已开发出一种DNA转染方案，该方案利用一种合成阳离子脂质，即N-[1-(2,3-二油酰氧基)丙基]-N,N,N-三甲基氯化铵（DOTMA）。含有DOTMA的小单层脂质体可自发地与DNA相互作用，形成脂质-DNA复合物，使DNA的包封率达到100%，DOTMA有助于复合物与组织培养细胞质膜融合，从而导致DNA的摄取和表达。该技术简单、重复性高，对于转染DNA的瞬时和稳定表达均有效。根据细胞系的不同，脂质转染比磷酸钙或DEAE-葡聚糖转染技术的效率高5至100倍以上。
Zotero 插件